Anti-TOP1MT Antibody (CAB15559)

Antibody Name:	Anti-TOP1MT Antibody
Antibody SKU:	CAB15559
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Rat
Positive Samples:	A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 470-550 of human TOP1MT (NP_443195.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	RATP STFE KSMQ NLQT KIQA KKEQ VAEA RAEL RRAR AEHK AQGD GKSR SVLE KKRR LLEK LQEQ LAQL SVQA TDKE ENKQ V
Gene ID:	116447
Uniprot:	Q969P6
Cellular Location:	Mitochondrion
Calculated MW:	58kDa/69kDa
Observed MW:	70kDa

Synonyms:

TOP1MT

Background:

This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

UniProt Protein Function:	Releases the supercoiling and torsional tension of DNA introduced during duplication of mitochondrial DNA by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.
NCBI Summary:	This gene encodes a mitochondrial DNA topoisomerase that plays a role in the modification of DNA topology. The encoded protein is a type IB topoisomerase and catalyzes the transient breaking and rejoining of DNA to relieve tension and DNA supercoiling generated in the mitochondrial genome during replication and transcription. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]
UniProt Code:	Q969P6
NCBI GenInfo Identifier:	386642871
NCBI Gene ID:	116447
NCBI Accession:	NP_001245375.1
UniProt Secondary Accession:	Q969P6,Q86ST4, Q86V82, B7ZAR5, E7ES89,
UniProt Related Accession:	Q969P6
Molecular Weight:	66
NCBI Full Name:	DNA topoisomerase I, mitochondrial isoform 2
NCBI Synonym Full Names:	DNA topoisomerase I mitochondrial
NCBI Official Symbol:	TOP1MT
NCBI Protein Information:	DNA topoisomerase I, mitochondrial
UniProt Protein Name:	DNA topoisomerase I, mitochondrial
UniProt Gene Name:	TOP1MT

	Western blot analysis of extracts of A-549 cells, using TOP1MT antibody (CAB15559) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60S.
	Immunohistochemistry of paraffin-embedded Human colon using TOP1MT Rabbit pAb (CAB15559) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat lung using TOP1MT Rabbit pAb (CAB15559) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)