Anti-TPI1 Antibody (CAB15733)

Antibody Name:	Anti-TPI1 Antibody
Antibody SKU:	CAB15733
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, 293T, DU145, A-431, Mouse stomach, Mouse brain, Mouse heart, Mouse kidnay, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-249 of human TPI1 (NP_000356.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAPS RKFF VGGN WKMN GRKQ SLGE LIGT LNAA KVPA DTEV VCAP PTAY IDFA RQKL DPKI AVAA QNCY KVTN GAFT GEIS PGMI KDCG ATWV VLGH SERR HVFG ESDE LIGQ KVAH ALAE GLGV IACI GEKL DERE AGIT EKVV FEQT KVIA DNVK DWSK VVLA YEPV WAIG TGKT ATPQ QAQE VHEK LRGW LKSN VSDA VAQS TRII YGGS VTGA TCKE LASQ PDVD GFLV GGAS LKPE FVDI INAK Q
Gene ID:	7167
Uniprot:	P60174
Cellular Location:
Calculated MW:	17kDa/26kDa/30kDa
Observed MW:	30kDa

Synonyms:

TPI1, HEL-S-49, TIM, TPI, TPID

Background:

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter.
UniProt Protein Details:	Protein type:EC 5.3.1.1; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; Carbohydrate Metabolism - inositol phosphate; Carbohydrate Metabolism - fructose and mannose Chromosomal Location of Human Ortholog: 12p13 Cellular Component: extracellular space; cytosol; nucleus Molecular Function:protein binding; triose-phosphate isomerase activity Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis Disease: Triosephosphate Isomerase Deficiency
NCBI Summary:	This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
UniProt Code:	P60174
NCBI GenInfo Identifier:	353526311
NCBI Gene ID:	7167
NCBI Accession:	P60174.3
UniProt Secondary Accession:	P60174,P00938, Q6FHP9, Q6IS07, Q8WWD0, Q96AG5, B7Z5D8 D3DUS9,
UniProt Related Accession:	P60174
Molecular Weight:	249
NCBI Full Name:	Triosephosphate isomerase
NCBI Synonym Full Names:	triosephosphate isomerase 1
NCBI Official Symbol:	TPI1
NCBI Official Synonym Symbols:	TIM; TPI; TPID; HEL-S-49
NCBI Protein Information:	triosephosphate isomerase; triose-phosphate isomerase; epididymis secretory protein Li 49
UniProt Protein Name:	Triosephosphate isomerase
UniProt Synonym Protein Names:	Triose-phosphate isomerase
UniProt Gene Name:	TPI1
UniProt Entry Name:	TPIS_HUMAN

	Western blot analysis of extracts of various cell lines, using TPI1 antibody (CAB15733) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded rat kidney using TPI1 antibody (CAB15733) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using TPI1 antibody (CAB15733) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse kidney using TPI1 antibody (CAB15733) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U-2 OS cells using TPI1 antibody (CAB15733) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-TPI1 Antibody (CAB2579)	Human Triosephosphate isomerase / TPI1 ELISA Kit
TPI1 Antibody (PACO12862)	Human Triosephosphate isomerase (TPI1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)