Anti-TPSAB1 Antibody (CAB2022)

Antibody Name:	Anti-TPSAB1 Antibody
Antibody SKU:	CAB2022
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-210 of human TPSAB1 (NP_003285.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:20 - 1:50
Reactivity:	Human, Mouse
Positive Samples:	M21, HepG2, NCI-H460, Mouse brain, Mouse heart, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 19-210 of human TPSAB1 (NP_003285.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	APAP GQAL QRVG IVGG QEAP RSKW PWQV SLRV HGPY WMHF CGGS LIHP QWVL TAAH CVGP DVKD LAAL RVQL REQH LYYQ DQLL PVSR IIVH PQFY TAQI GADI ALLE LEEP VNVS SHVH TVTL PPAS ETFP PGMP CWVT GWGD VDND ERLP PPFP LKQV KVPI MENH ICDA KYHL GAYT GDDV RIVR DDML
Gene ID:	7177
Uniprot:	Q15661
Cellular Location:	Secreted
Calculated MW:	29kDa/30kDa
Observed MW:	31kDa

Synonyms:

TPSAB1, TPS1, TPS2, TPSB1

Background:

Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders.

UniProt Protein Function:	TPSAB1: Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. Belongs to the peptidase S1 family. Tryptase subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted, signal peptide; EC 3.4.21.59; Protease; Secreted Chromosomal Location of Human Ortholog: 16p13.3 Cellular Component: extracellular matrix; extracellular space; extracellular region Molecular Function:protein binding; serine-type peptidase activity; serine-type endopeptidase activity Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; defense response; proteolysis
NCBI Summary:	Tryptases comprise a family of trypsin-like serine proteases, the peptidase family S1. Tryptases are enzymatically active only as heparin-stabilized tetramers, and they are resistant to all known endogenous proteinase inhibitors. Several tryptase genes are clustered on chromosome 16p13.3. These genes are characterized by several distinct features. They have a highly conserved 3' UTR and contain tandem repeat sequences at the 5' flank and 3' UTR which are thought to play a role in regulation of the mRNA stability. These genes have an intron immediately upstream of the initiator Met codon, which separates the site of transcription initiation from protein coding sequence. This feature is characteristic of tryptases but is unusual in other genes. The alleles of this gene exhibit an unusual amount of sequence variation, such that the alleles were once thought to represent two separate genes, alpha and beta 1. Beta tryptases appear to be the main isoenzymes expressed in mast cells; whereas in basophils, alpha tryptases predominate. Tryptases have been implicated as mediators in the pathogenesis of asthma and other allergic and inflammatory disorders. [provided by RefSeq, Jul 2008]
UniProt Code:	Q15661
NCBI GenInfo Identifier:	18202508
NCBI Gene ID:	7177
NCBI Accession:	Q15661.1
UniProt Secondary Accession:	Q15661,P15157, Q15663, Q6B052, Q9H2Y4, Q9H2Y5, Q9UQI1 D2E6R9, D2E6S1,
UniProt Related Accession:	Q15661
Molecular Weight:	Observed MW: 31kDaCalculated MW: 29kDa/30kDa
NCBI Full Name:	Tryptase alpha/beta-1
NCBI Synonym Full Names:	tryptase alpha/beta 1
NCBI Official Symbol:	TPSAB1
NCBI Official Synonym Symbols:	TPS1; TPS2; TPSB1
NCBI Protein Information:	tryptase alpha/beta-1; tryptase I; tryptase-1; tryptase-I; tryptase-III; tryptase beta 1; tryptase beta I; tryptase beta-1; tryptase alpha-1; tryptase alpha II; mast cell beta I tryptase; mast cell alpha II tryptase
UniProt Protein Name:	Tryptase alpha/beta-1
UniProt Synonym Protein Names:	Tryptase I; Tryptase alpha-1
Protein Family:	Tryptase
UniProt Gene Name:	TPSAB1
UniProt Entry Name:	TRYB1_HUMAN

	Western blot analysis of extracts of various cell lines, using TPSAB1 antibody (CAB2022) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of A-549 cells using TPSAB1 antibody (CAB2022). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-TPSAB1 Antibody (CAB14471)	Human Tryptase alpha / TPS1 ELISA Kit
TPSAB1 Antibody (PACO12874)	Human Tryptase alpha/beta-1 (TPSAB1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)