Anti-TRIM28 Antibody (CAB2245)

Antibody Name:	Anti-TRIM28 Antibody
Antibody SKU:	CAB2245
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 576-835 of human TRIM28 (NP_005753.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:1000 - 1:3000 IHC 1:100 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, HT-29, SKOV3, A-549, MCF7, Mouse liver, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 576-835 of human TRIM28 (NP_005753.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PVLM ALAE GPGA EGPR LASP SGST SSGL EVVA PEGT SAPG GGPG TLDD SATI CRVC QKPG DLVM CNQC EFCF HLDC HLPA LQDV PGEE WSCS LCHV LPDL KEED GSLS LDGA DSTG VVAK LSPA NQRK CERV LLAL FCHE PCRP LHQL ATDS TFSL DQPG GTLD LTLI RARL QEKL SPPY SSPQ EFAQ DVGR MFKQ FNKL TEDK ADVQ SIIG LQRF FETR MNEA FGDT KFSA VLVE PPPM SLPG AGLS SQEL SGGP GDGP
Gene ID:	10155
Uniprot:	Q13263
Cellular Location:	Nucleus
Calculated MW:	79kDa/88kDa
Observed MW:	120kDa

Synonyms:

KAP1, PPP1R157, RNF96, TF1B, TIF1B, TRIM28

Background:

The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

UniProt Protein Function:	TIF1B: an atypical protein kinase that functions as a transcriptional coactivator. Mediates transcriptional regulation by interaction with the Kruppel-associated box (KRAB) repression domain found in many transcription factors. Binds to the negative regulatory domain of c-Myb and negatively regulates the c-Myb-dependent trans-activation. Localizes to the nucleus and is thought to associate with specific chromatin regions. Phosphorylated in an ATM-dependent manner following double-strand breaks. Its phosphorylation in response to DNA damage leads to chromatin relaxation. A member of the tripartite motif family. The tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
UniProt Protein Details:	Protein type:EC 6.3.2.-; Ligase; Protein kinase, atypical; Ubiquitin conjugating system; Kinase, protein; Ubiquitin ligase; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; ATYPICAL group; TIF1 family Chromosomal Location of Human Ortholog: 19q13.4 Cellular Component: nucleoplasm; nuclear heterochromatin; nucleus Molecular Function:protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; ubiquitin protein ligase binding; transcription coactivator activity; ubiquitin-protein ligase activity; transcription factor activity; transcription corepressor activity; ligase activity; protein kinase activity Biological Process: transcription initiation from RNA polymerase II promoter; convergent extension involved in axis elongation; positive regulation of DNA repair; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; protein ubiquitination; negative regulation of transcription from RNA polymerase II promoter; DNA repair; protein oligomerization; protein sumoylation; positive regulation of transcription factor import into nucleus; innate immune response; epithelial to mesenchymal transition; gene expression; negative regulation of transcription, DNA-dependent; DNA methylation during embryonic development; embryo implantation
NCBI Summary:	The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13263
NCBI GenInfo Identifier:	3183179
NCBI Gene ID:	10155
NCBI Accession:	Q13263.5
UniProt Secondary Accession:	Q13263,O00677, Q7Z632, Q93040, Q96IM1,
UniProt Related Accession:	Q13263
Molecular Weight:	835
NCBI Full Name:	Transcription intermediary factor 1-beta
NCBI Synonym Full Names:	tripartite motif containing 28
NCBI Official Symbol:	TRIM28
NCBI Official Synonym Symbols:	KAP1; TF1B; RNF96; TIF1B; PPP1R157
NCBI Protein Information:	transcription intermediary factor 1-beta; KAP-1; KRIP-1; TIF1-beta; RING finger protein 96; KRAB-associated protein 1; nuclear corepressor KAP-1; KRAB-interacting protein 1; E3 SUMO-protein ligase TRIM28; tripartite motif-containing 28; tripartite motif-c
UniProt Protein Name:	Transcription intermediary factor 1-beta
UniProt Synonym Protein Names:	E3 SUMO-protein ligase TRIM28 (EC:6.3.2.-); KRAB-associated protein 1; KAP-1; KRAB-interacting protein 1; KRIP-1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28
UniProt Gene Name:	TRIM28
UniProt Entry Name:	TIF1B_HUMAN

	Western blot analysis of extracts of various cell lines, using TRIM28 antibody (CAB2245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat brain using TRIM28 antibody (CAB2245) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using TRIM28 Polyclonal Antibody (CAB2245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using TRIM28 Polyclonal Antibody (CAB2245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using TRIM28 Polyclonal Antibody (CAB2245) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-KAP1 Antibody (CAB19568)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)