Anti-TRIM63 Antibody (CAB3101)

Antibody Name:	Anti-TRIM63 Antibody
Antibody SKU:	CAB3101
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human TRIM63

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse heart, Rat heart

Immunogen:	A synthetic peptide of human TRIM63
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	84676
Uniprot:	Q969Q1
Cellular Location:	Cytoplasm, M line, Nucleus, Z line, myofibril, sarcomere
Calculated MW:	36kDa/40kDa
Observed MW:	40kDa

Synonyms:

TRIM63, IRF, MURF1, MURF2, RNF28, SMRZ

Background:

This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase.

UniProt Protein Function:	MURF1: E3 ubiquitin ligase. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric- associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
UniProt Protein Details:	Protein type:EC 6.3.2.-; Ligase; EC 6.3.2.19; Ubiquitin ligase; Ubiquitin conjugating system Chromosomal Location of Human Ortholog: 1p34-p33 Cellular Component: microtubule; cytoplasm; M band; Z disc; nucleus Molecular Function:signal transducer activity; protein binding; zinc ion binding; ubiquitin-protein ligase activity; titin binding; ligase activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; skeletal muscle atrophy; muscle contraction; regulation of gene expression; protein ubiquitination; response to electrical stimulus involved in regulation of muscle adaptation; signal transduction
NCBI Summary:	This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]
UniProt Code:	Q969Q1
NCBI GenInfo Identifier:	21362898
NCBI Gene ID:	84676
NCBI Accession:	Q969Q1.1
UniProt Related Accession:	Q969Q1
Molecular Weight:	40.2kDa
NCBI Full Name:	E3 ubiquitin-protein ligase TRIM63
NCBI Synonym Full Names:	tripartite motif containing 63
NCBI Official Symbol:	TRIM63
NCBI Official Synonym Symbols:	IRF; SMRZ; MURF1; MURF2; RNF28
NCBI Protein Information:	E3 ubiquitin-protein ligase TRIM63
UniProt Protein Name:	E3 ubiquitin-protein ligase TRIM63
UniProt Synonym Protein Names:	Iris RING finger protein; Muscle-specific RING finger protein 1; MuRF-1; MuRF1; RING finger protein 28; Striated muscle RING zinc finger protein; Tripartite motif-containing protein 63
UniProt Gene Name:	TRIM63
UniProt Entry Name:	TRI63_HUMAN

	Western blot analysis of extracts of various cell lines, using TRIM63 antibody (CAB3101) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of A549 cells using TRIM63 antibody (CAB3101). Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)