Anti-TRIP4 Antibody (CAB15352)

Antibody Name:	Anti-TRIP4 Antibody
Antibody SKU:	CAB15352
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 282-581 of human TRIP4 (NP_057297.2).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, HeLa, LO2, Mouse liver, Mouse testis, Mouse brain, Rat testis, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 282-581 of human TRIP4 (NP_057297.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	VIDD ESDY FASD SNQW LSKL ERET LQKR EEEL RELR HASR LSKK VTID FAGR KILE EENS LAEY HSRL DETI QAIA NGTL NQPL TKLD RSSE EPLG VLVN PNMY QSPP QWVD HTGA ASQK KAFR SSGF GLEF NSFQ HQLR IQDQ EFQE GFDG GWCL SVHQ PWAS LLVR GIKR VEGR SWYT PHRG RLWI AATA KKPS PQEV SELQ ATYR LLRG KDVE FPND YPSG CLLG CVDL IDCL SQKQ FKEQ FPDI SQES DSPF VFIC KNPQ EMVV KFPI KGNP KIWK LDSK IHQG AKKG LMKQ NKAV
Gene ID:	9325
Uniprot:	Q15650
Cellular Location:	Cytoplasm, Nucleus, centrosome, cytoskeleton, cytosol, microtubule organizing center
Calculated MW:	66kDa
Observed MW:	66kDa

Synonyms:

TRIP4, ASC-1, ASC1, HsT17391, MDCDC, SMABF1, ZC2HC5

Background:

This gene encodes a subunit of the tetrameric nuclear activating signal cointegrator 1 (ASC-1) complex, which associates with transcriptional coactivators, nuclear receptors and basal transcription factors to facilitate nuclear receptors-mediated transcription. This protein is localized in the nucleus and contains an E1A-type zinc finger domain, which mediates interaction with transcriptional coactivators and ligand-bound nuclear receptors, such as thyroid hormone receptor and retinoid X receptor alpha, but not glucocorticoid receptor. Mutations in this gene are associated with spinal muscular atrophy with congenital bone fractures-1 (SMABF1).

UniProt Protein Function:	TRIP4: thyroid receptor interacting protein 4 is a transcription coactivator of nuclear receptors including thyroid hormone receptor. Functions in conjunction with CBP-p300 and SRC-1. Plays a pivotal role in the transactivation of NF-kappa-B, SRF and AP1. Acts as a mediator of transrepression between nuclear receptor and either AP1 or NF-kappa-B. Plays a role in androgen receptor transactivation and in testicular function
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 15q22.31 Cellular Component: cytoplasm; cytosol; microtubule organizing center; nucleoplasm; nucleus Molecular Function:estrogen receptor binding; histone acetyltransferase binding; ligand-dependent nuclear receptor binding; protease binding; protein binding; transcription coactivator activity; zinc ion binding Biological Process: estrogen receptor signaling pathway; positive regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter
NCBI Summary:	This gene encodes a subunit of the tetrameric nuclear activating signal cointegrator 1 (ASC-1) complex, which associates with transcriptional coactivators, nuclear receptors and basal transcription factors to facilitate nuclear receptors-mediated transcription. This protein is localized in the nucleus and contains an E1A-type zinc finger domain, which mediates interaction with transcriptional coactivators and ligand-bound nuclear receptors, such as thyroid hormone receptor and retinoid X receptor alpha, but not glucocorticoid receptor. Mutations in this gene are associated with spinal muscular atrophy with congenital bone fractures-1 (SMABF1). [provided by RefSeq, Apr 2016]
UniProt Code:	Q15650
NCBI GenInfo Identifier:	116242828
NCBI Gene ID:	9325
NCBI Accession:	Q15650.4
UniProt Secondary Accession:	Q15650,Q96ED7, Q9UKH0, B2RAS0,
UniProt Related Accession:	Q15650
Molecular Weight:	66,146 Da
NCBI Full Name:	Activating signal cointegrator 1
NCBI Synonym Full Names:	thyroid hormone receptor interactor 4
NCBI Official Symbol:	TRIP4
NCBI Official Synonym Symbols:	ASC1; ASC-1; SMABF1; ZC2HC5; HsT17391
NCBI Protein Information:	activating signal cointegrator 1
UniProt Protein Name:	Activating signal cointegrator 1
UniProt Synonym Protein Names:	Thyroid receptor-interacting protein 4
Protein Family:	Activating signal cointegrator
UniProt Gene Name:	TRIP4
UniProt Entry Name:	TRIP4_HUMAN

	Western blot analysis of extracts of various cell lines, using TRIP4 antibody (CAB15352) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded rat heart using TRIP4 antibody (CAB15352) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using TRIP4 antibody (CAB15352) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human esophageal using TRIP4 antibody (CAB15352) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of A-549 cells using 3ug TRIP4 antibody (CAB15352). Western blot was performed from the immunoprecipitate using TRIP4 antibody (CAB15352) at a dilition of 1:1000.

Antibodies

TRIP4 Antibody (PACO05745)

TRIP4 Antibody (PACO12928)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)