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Anti-TUFM Antibody (CAB6423)

SKU:
CAB6423
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 2 Anti-TUFM Antibody CAB6423
  • Immunology Antibodies 2 Anti-TUFM Antibody CAB6423
  • Immunology Antibodies 2 Anti-TUFM Antibody CAB6423
  • Immunology Antibodies 2 Anti-TUFM Antibody CAB6423
€139 - €419
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Description

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Antibody Name:Anti-TUFM Antibody
Antibody SKU:CAB6423
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human TUFM (NP_003312.3).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-29, HepG2, HeLa, A-431, Jurkat, Mouse kidney, Mouse liver, Rat kidney, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human TUFM (NP_003312.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:ADAV QDSE MVEL VELE IREL LTEF GYKG EETP VIVG SALC ALEG RDPE LGLK SVQK LLDA VDTY IPVP ARDL EKPF LLPV EAVY SVPG RGTV VTGT LERG ILKK GDEC ELLG HSKN IRTV VTGI EMFH KSLE RAEA GDNL GALV RGLK REDL RRGL VMVK PGSI KPHQ KVEA QVYI LSKE EGGR HKPF VSHF MPVM FSLT WDMA CRII LPPE KELA MPGE DLKF NLIL RQPM ILEK GQRF TLRD GNRT IGTG LVTN TLAM TEEE KNIK WG
Gene ID:7284
Uniprot:P49411
Cellular Location:Mitochondrion
Calculated MW:49kDa
Observed MW:48kDa
Synonyms:TUFM, COXPD4, EF-TuMT, EFTU, P43
Background:This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17.
UniProt Protein Function:EFTU: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Defects in TUFM are the cause of combined oxidative phosphorylation deficiency type 4 (COXPD4). COXPD4 is characterized by neonatal lactic acidosis, rapidly progressive encephalopathy, severely decreased mitochondrial protein synthesis, and combined deficiency of mtDNA-related mitochondrial respiratory chain complexes. Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
UniProt Protein Details:

Protein type:Translation elongation; RNA-binding; Translation; Mitochondrial

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: membrane; mitochondrion

Molecular Function:GTP binding; GTPase activity; protein binding; translation elongation factor activity

Biological Process: mitochondrial translation; organelle organization and biogenesis; translational elongation

Disease: Combined Oxidative Phosphorylation Deficiency 4

NCBI Summary:This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17. [provided by RefSeq, Jul 2008]
UniProt Code:P49411
NCBI GenInfo Identifier:1706611
NCBI Gene ID:7284
NCBI Accession:P49411.2
UniProt Secondary Accession:P49411,O15276,
UniProt Related Accession:P49411
Molecular Weight:
NCBI Full Name:Elongation factor Tu, mitochondrial
NCBI Synonym Full Names:Tu translation elongation factor, mitochondrial
NCBI Official Symbol:TUFM  
NCBI Official Synonym Symbols:P43; EFTU; COXPD4; EF-TuMT  
NCBI Protein Information:elongation factor Tu, mitochondrial
UniProt Protein Name:Elongation factor Tu, mitochondrial
UniProt Synonym Protein Names:P43
UniProt Gene Name:TUFM  
UniProt Entry Name:EFTU_HUMAN
Anti-TUFM Antibody (CAB6423)Western blot analysis of extracts of various cell lines, using TUFM antibody (CAB6423) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-TUFM Antibody (CAB6423)Immunohistochemistry of paraffin-embedded human esophageal cancer using TUFM antibody (CAB6423) at dilution of 1:100 (40x lens).
Anti-TUFM Antibody (CAB6423)Immunofluorescence analysis of MCF7 cells using TUFM antibody (CAB6423). Blue: DAPI for nuclear staining.
Anti-TUFM Antibody (CAB6423)Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug TUFM antibody (CAB6423). Western blot was performed from the immunoprecipitate using TUFM antibody (CAB6423) at a dilition of 1:1000.

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