Anti-Twist Antibody (CAB3237)
- SKU:
- CAB3237
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-Twist Antibody |
| Antibody SKU: | CAB3237 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Twist (NP_000465.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | mouse brain, rat brain, A-549, U-87MG, SGC-7901, NIH/3T3 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Twist (NP_000465.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MMQD VSSS PVSP ADDS LSNS EEEP DRQQ PPSG KRGG RKRR SSRR SAGG GAGP GGAA GGGV GGGD EPGS PAQG KRGK KSAG CGGG GGAG GGGG SSSG GGSP |
| Gene ID: | 7291 |
| Uniprot: | Q15672 |
| Cellular Location: | Nucleus |
| Calculated MW: | 21kDa |
| Observed MW: | 21kDa |
| Synonyms: | TWIST1, ACS3, BPES2, BPES3, CRS, CRS1, CSO, SCS, TWIST, bHLHa38 |
| Background: | Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. |
| UniProt Protein Function: | TWIST1: Acts as a transcriptional regulator. Inhibits myogenesis by sequestrating E proteins, inhibiting trans-activation by MEF2, and inhibiting DNA-binding by MYOD1 through physical interaction. This interaction probably involves the basic domains of both proteins. Also represses expression of proinflammatory cytokines such as TNFA and IL1B. Regulates cranial suture patterning and fusion. Activates transcription as a heterodimer with E proteins. Regulates gene expression differentially, depending on dimer composition. Homodimers induce expression of FGFR2 and POSTN while heterodimers repress FGFR2 and POSTN expression and induce THBS1 expression. Heterodimerization is also required for osteoblast differentiation. Efficient DNA binding requires dimerization with another bHLH protein. Homodimer or heterodimer with E proteins such as TCF3. ID1 binds preferentially to TCF3 but does not interact efficiently with TWIST1 so ID1 levels control the amount of TCF3 available to dimerize with TWIST1 and thus determine the type of dimer formed. Subset of mesodermal cells. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription regulation; Inhibitor; Cell development/differentiation Chromosomal Location of Human Ortholog: 7p21.2 Cellular Component: nucleus Molecular Function:protein domain specific binding; protein binding; protein homodimerization activity; protein heterodimerization activity; bHLH transcription factor binding; transcription factor binding Biological Process: transcription from RNA polymerase II promoter; embryonic forelimb morphogenesis; negative regulation of skeletal muscle development; muscle development; rhythmic process; neuron migration; palate development; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; negative regulation of histone phosphorylation; negative regulation of histone acetylation; odontogenesis; negative regulation of DNA damage response, signal transduction by p53 class mediator; negative regulation of osteoblast differentiation; ossification; in utero embryonic development; negative regulation of transcription factor activity; outer ear morphogenesis; embryonic cranial skeleton morphogenesis; negative regulation of tumor necrosis factor production; positive regulation of tumor necrosis factor production; positive regulation of fatty acid beta-oxidation; osteoblast differentiation; positive regulation of angiogenesis; neural tube closure; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of transcription, DNA-dependent; regulation of bone mineralization; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of phosphoinositide 3-kinase cascade Disease: Robinow-sorauf Syndrome; Craniosynostosis 1; Saethre-chotzen Syndrome |
| NCBI Summary: | Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. The protein encoded by this gene is a bHLH transcription factor and shares similarity with another bHLH transcription factor, Dermo1. The strongest expression of this mRNA is in placental tissue; in adults, mesodermally derived tissues express this mRNA preferentially. Mutations in this gene have been found in patients with Saethre-Chotzen syndrome. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q15672 |
| NCBI GenInfo Identifier: | 2498009 |
| NCBI Gene ID: | 7291 |
| NCBI Accession: | Q15672.1 |
| UniProt Secondary Accession: | Q15672,Q92487, Q99804, A4D128, |
| UniProt Related Accession: | Q15672 |
| Molecular Weight: | Calculated: 20 kdaObserved: 34 kDa |
| NCBI Full Name: | Twist-related protein 1 |
| NCBI Synonym Full Names: | twist family bHLH transcription factor 1 |
| NCBI Official Symbol: | TWIST1Â Â |
| NCBI Official Synonym Symbols: | CRS; CSO; SCS; ACS3; CRS1; BPES2; BPES3; TWIST; bHLHa38Â Â |
| NCBI Protein Information: | twist-related protein 1; H-twist; twist homolog 1; B-HLH DNA binding protein; TWIST homolog of drosophila; class A basic helix-loop-helix protein 38; twist basic helix-loop-helix transcription factor 1 |
| UniProt Protein Name: | Twist-related protein 1 |
| UniProt Synonym Protein Names: | Class A basic helix-loop-helix protein 38; bHLHa38; H-twist |
| Protein Family: | Twist-related protein |
| UniProt Gene Name: | TWIST1Â Â |
| UniProt Entry Name: | TWST1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Twist antibody (CAB3237) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1min. |
 | Western blot analysis of extracts of various cell lines, using Twist antibody (CAB3237) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat brain using Twist Rabbit pAb (CAB3237) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using Twist Rabbit pAb (CAB3237) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using Twist Rabbit pAb (CAB3237) at dilution of 1:200 (40x lens). |
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