Anti-TXNRD1 Antibody (CAB16631)

Antibody Name:	Anti-TXNRD1 Antibody
Antibody SKU:	CAB16631
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant protein of human TXNRD1

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HepG2, BxPC-3, Mouse heart

Immunogen:	Recombinant protein of human TXNRD1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	7296
Uniprot:	Q16881
Cellular Location:
Calculated MW:	50kDa/54kDa/59kDa/60kDa/65kDa/67kDa/70kDa
Observed MW:	65kDa

Synonyms:

TXNRD1, GRIM-12, TR, TR1, TRXR1, TXNR

Background:

This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms.

UniProt Protein Function:	TRXR1: a cytoplasmic pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine residue which is required for the catalytic activity.
UniProt Protein Details:	Protein type:EC 1.8.1.9; Nuclear receptor co-regulator; Oxidoreductase; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: 12q23-q24.1 Cellular Component: nucleoplasm; nucleolus; cytosol Molecular Function:protein binding; thioredoxin-disulfide reductase activity; FAD binding; electron carrier activity; protein disulfide oxidoreductase activity Biological Process: response to reactive oxygen species; cell redox homeostasis; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; cellular lipid metabolic process; signal transduction
NCBI Summary:	This gene encodes a member of the family of pyridine nucleotide oxidoreductases. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. The functional enzyme is thought to be a homodimer which uses FAD as a cofactor. Each subunit contains a selenocysteine (Sec) residue which is required for catalytic activity. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenocysteine-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing results in several transcript variants encoding the same or different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	Q16881
NCBI GenInfo Identifier:	172046253
NCBI Gene ID:	7296
NCBI Accession:	Q16881.3
UniProt Secondary Accession:	Q16881,Q6FI31, Q6VB40, Q6VB41, Q6VB42, Q6VBP2, Q6VBP3 B7Z1F4, B7Z3Y8, B7Z904, E9PMY9, F5H780,
UniProt Related Accession:	Q16881
Molecular Weight:	50,873 Da
NCBI Full Name:	Thioredoxin reductase 1, cytoplasmic
NCBI Synonym Full Names:	thioredoxin reductase 1
NCBI Official Symbol:	TXNRD1
NCBI Official Synonym Symbols:	TR; TR1; TXNR; TRXR1; GRIM-12
NCBI Protein Information:	thioredoxin reductase 1, cytoplasmic; oxidoreductase; thioredoxin reductase TR1; thioredoxin reductase GRIM-12; KM-102-derived reductase-like factor; gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein
UniProt Protein Name:	Thioredoxin reductase 1, cytoplasmic
UniProt Synonym Protein Names:	Gene associated with retinoic and interferon-induced mortality 12 protein; GRIM-12; Gene associated with retinoic and IFN-induced mortality 12 protein; KM-102-derived reductase-like factor; Thioredoxin reductase TR1
Protein Family:	Tropinone reductase
UniProt Gene Name:	TXNRD1
UniProt Entry Name:	TRXR1_HUMAN

	Western blot analysis of extracts of various cell lines, using TXNRD1 antibody (CAB16631) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human placenta using TXNRD1 antibody (CAB16631) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using TXNRD1 antibody (CAB16631) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using TXNRD1 antibody (CAB16631) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using TXNRD1 antibody (CAB16631) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-TXNRD1 Antibody (CAB4725)	Human TrxR1 (Thioredoxin Reductase 1) CLIA Kit (HUES00966)
TXNRD1 Antibody (PACO13004)	Human Thioredoxin reductase 1, cytoplasmic (TXNRD1) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)