Anti-UBE2A Antibody (CAB12528)

Antibody Name:	Anti-UBE2A Antibody
Antibody SKU:	CAB12528
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2A (NP_003327.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSTP ARRR LMRD FKRL QEDP PAGV SGAP SENN IMVW NAVI FGPE GTPF EDGT FKLT IEFT EEYP NKPP TVRF VSKM FHPN VYAD GSIC LDIL QNRW SPTY DVSS ILTS IQSL LDEP NPNS PANS QAAQ LYQE NKRE YEKR VSAI VEQS WRDC
Gene ID:	7319
Uniprot:	P49459
Cellular Location:
Calculated MW:	8kDa/13kDa/17kDa
Observed MW:	17kDa

Synonyms:

UBE2A, HHR6A, MRXS30, MRXSN, RAD6A, UBC2

Background:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with mental retardation. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	UBE2A: a ubiquitin-protein ligase, a member of the ubiquitin-conjugating enzyme family. Homologous to the yeast DNA repair gene RAD6. Interacts with RAD18. Required for postreplication repair of UV-damaged DNA.
UniProt Protein Details:	Protein type:EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase; Ligase Chromosomal Location of Human Ortholog: Xq24 Cellular Component: XY body; cytoplasm; nuclear chromatin; chromatin; cytosol Molecular Function:protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of peptide antigen via MHC class I; protein autoubiquitination; protein polyubiquitination; maternal process involved in pregnancy; postreplication repair; in utero embryonic development; positive regulation of cell proliferation; DNA repair; histone H2A ubiquitination; response to UV Disease: Mental Retardation, X-linked, Syndromic, Nascimento Type
UniProt Code:	P49459
NCBI GenInfo Identifier:	33518639
NCBI Gene ID:	7319
NCBI Accession:	P49459.2
Molecular Weight:
NCBI Full Name:	Ubiquitin-conjugating enzyme E2 A
UniProt Protein Name:	Ubiquitin-conjugating enzyme E2 A
UniProt Synonym Protein Names:	RAD6 homolog A; HR6A; hHR6A; Ubiquitin carrier protein A; Ubiquitin-protein ligase A
Protein Family:	Ubiquitin-conjugating enzyme
UniProt Gene Name:	UBE2A
UniProt Entry Name:	UBE2A_HUMAN

	Western blot analysis of extracts of mouse kidney, using UBE2A antibody (CAB12528) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunofluorescence analysis of U2OS cells using UBE2A antibody (CAB12528) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-UBE2A Antibody (CAB7744)	Human Ubiquitin-conjugating enzyme E2 A / UBE2A ELISA Kit
UBE2A Antibody (PACO13020)	Human UBE2A (Ubiquitin Conjugating Enzyme E2A) CLIA Kit (HUES01229)
	Human Ubiquitin-conjugating enzyme E2 A (UBE2A) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)