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Anti-UBL4A Antibody (CAB4211)

SKU:
CAB4211
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
  • Signal Transduction Antibodies 2 Anti-UBL4A Antibody CAB4211
  • Signal Transduction Antibodies 2 Anti-UBL4A Antibody CAB4211
€139 - €419
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Description

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Antibody Name:Anti-UBL4A Antibody
Antibody SKU:CAB4211
Antibody Size:20uL, 50uL, 100uL
Application:WB IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human UBL4A (NP_055050.1).
Application:WB IP
Recommended Dilution:WB 1:200 - 1:1000 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, A-549, U-87MG, Mouse brain, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of human UBL4A (NP_055050.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MQLT VKAL QGRE CSLQ VPED ELVS TLKQ LVSE KLNV PVRQ QRLL FKGK ALAD GKRL SDYS IGPN SKLN LVVK PLEK VLLE EGEA QRLA DSPP PQVW QLIS KVLA RHFS AADA SRVL EQLQ RDYE RSLS RLTL DDIE RLAS RFLH PEVT ETME KGFS K
Gene ID:8266
Uniprot:P11441
Cellular Location:Cytoplasm, cytosol
Calculated MW:17kDa
Observed MW:14-16kDa
Synonyms:UBL4A, DX254E, DXS254E, G6PD, GDX, GET5, MDY2, TMA24, UBL4
Background:
UniProt Protein Function:UBL4A: Component of the BAT3 complex, a multiprotein complex involved in the post-translational delivery of tail-anchored (TA) membrane proteins to the endoplasmic reticulum membrane. TA membrane proteins, also named type II transmembrane proteins, contain a single C-terminal transmembrane region. The complex acts by facilitating TA proteins capture by ASNA1/TRC40: it is recruited to ribosomes synthesizing membrane proteins, interacts with the transmembrane region of newly released TA proteins, and transfers them to ASNA1/TRC40 for targeting.
UniProt Protein Details:

Protein type:Ubiquitin-like modifier

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: cytosol

Molecular Function:protein binding; small conjugating protein ligase activity

Biological Process: transport; protein modification process

UniProt Code:P11441
NCBI GenInfo Identifier:136662
NCBI Gene ID:8266
NCBI Accession:P11441.1
UniProt Secondary Accession:P11441,Q5HY80,
UniProt Related Accession:P11441
Molecular Weight:157
NCBI Full Name:Ubiquitin-like protein 4A
NCBI Synonym Full Names:ubiquitin-like 4A
NCBI Official Symbol:UBL4A  
NCBI Official Synonym Symbols:GDX; G6PD; GET5; MDY2; UBL4; TMA24; DX254E; DXS254E  
NCBI Protein Information:ubiquitin-like protein 4A; ubiquitin-like 4; ubiquitin-like protein GDX
UniProt Protein Name:Ubiquitin-like protein 4A
UniProt Synonym Protein Names:Ubiquitin-like protein GDX
Protein Family:Ubiquitin-like protein
UniProt Gene Name:UBL4A  
UniProt Entry Name:UBL4A_HUMAN
Anti-UBL4A Antibody (CAB4211)Western blot analysis of extracts of various cell lines, using UBL4A antibody (CAB4211) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-UBL4A Antibody (CAB4211)Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug UBL4A antibody (CAB4211). Western blot was performed from the immunoprecipitate using UBL4A antibody (CAB4211) at a dilition of 1:1000.

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