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Western blot analysis of various lysates, using UFC1 Rabbit mAb (CAB21154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Key Features

UFC1 Monoclonal Antibody (CAB21154)

Datasheet MSDS
SKU CAB21154
Product Type Antibody
Antibody Type Monoclonal Antibody
Reactivity Human, Mouse, Rat
Host Species Rabbit
Synonyms NEDSG, HSPC155, UFC1
$173 - $603

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Description

system_update_altDatasheetsystem_update_altMSDS

UFC1 Monoclonal Antibody (CAB21154)

The UFC1 Monoclonal Antibody (CAB21154) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced using state-of-the-art technology, specifically targets UFC1 in human samples and is validated for use in various experimental techniques, including Western blotting. By binding to UFC1, researchers can accurately detect and analyze the protein in a wide range of cell types, making it a versatile tool for studies in molecular biology and cell physiology.

This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:UFC1 Monoclonal Antibody
SKU:CAB21154
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Clone Number:ARC3001
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MADE ATRR VVSE IPVL KTNA GPRD RELW VQRL KEEY QSLI RYVE NNKN ADND WFRL ESNK EGTR WFGK CWYI HDLL KYEF DIEF DIPI TYPT TAPE IAVP ELDG KTAK MYRG GKIC LTDH FKPL WARN VP
Tested Applications:WB ELISA
Recommended Dilution:
WB1:1000 - 1:4000
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:NEDSG, HSPC155, UFC1
Positive Sample:HepG2, MCF7, THP-1, Mouse pancreas, Rat pancreas
Cellular Localization:Extracellular Exosome.
Calculated MW:19kDa
Observed MW:19kDa

UFC1 is an E2-like conjugating enzyme for ubiquitin-fold modifier-1 (UFM1; MIM 610553) (Komatsu et al., 2004 [PubMed 15071506]).

Purification MethodAffinity purification
Gene ID51506
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of various lysates, using UFC1 Rabbit mAb (CAB21154) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 20s.
Product ImageWestern blot analysis of various lysates, using UFC1 Rabbit mAb (CAB21154) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s.
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