Description
ULK1/ULK2 Antibody (CAB18685)
The ULK1/ULK2 Antibody (CAB18685) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, is highly reactive with ULK1 and ULK2 proteins, key regulators of autophagy initiation. Validation studies have shown that this antibody is suitable for use in Western blot applications, enabling accurate detection and analysis of ULK1 and ULK2 in various cell types. Its specificity for these proteins makes it an ideal choice for studies in cell biology, cancer research, and neurodegenerative diseases where autophagy dysregulation is implicated.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
| Product Name: | ULK1/ULK2 Antibody |
| SKU: | CAB18685 |
| Size: | 20μL, 100μL |
| Reactivity: | Human |
| Immunogen: | Recombinant protein (or fragment).This information is considered to be commercially sensitive. | ||||
| Sequence: | MEPG RGGT ETVG KFEF SRKD LIGH GAFA VVFK GRHR EKHD LEVA VKCI NKKN LAKS QTLL GKEI KILK ELKH ENIV ALYD FQEM ANSV YLVM EYCN GGDL ADYL HAMR TLSE DTIR LFLQ QIAG AMRL LHSK GIIH RDLK PQNI LLSN PAGR RANP NSIR VKIA DFGF ARYL QSNM MAAT LCGS PMYM APEV IMSQ HYDG KADL WSIG TIVY QCLT GKAP FQAS SPQD LRLF YEKN KTLV PTIP RETS APLR QLLL ALLQ RNHK DRMD FDEF FHHP FLDA SPS | ||||
| Tested Applications: | WB ELISA | ||||
| Recommended Dilution: |
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| Positive Sample: | RD, SH-SY5Y, HepG2 |
| Cellular Localization: | Atg1/Ulk1 Kinase Complex, Autophagosome, Cytoplasm, Cytosol, Mitochondrial Outer Membrane, Recycling Endosome. |
| Observed MW: | 113kDa |
Mammalian ULK1 (unc-51 like kinase 1) and ULK2, Caenorhabditis elegans UNC-51, and Drosophila melanogaster Atg1 are serine/threonine kinases that regulate flux through the autophagy pathway in response to various types of cellular stress.ULK1 and ULK2 all have different tissue-specific expressions partially contributing to diverse and ULK-specific interaction networks in various tissues.ULK1 (or its close family member ULK2) interacts with several other proteins to form a large complex that is required for the formation of an autophagosome. The other proteins that complex with ULK1/ULK2 include ATG13, ATG101, and RB1CC1 (FIP200/ATG17).AMPK, activated during low nutrient conditions, directly phophorylates ULK1 at multiple sites including Ser317, Ser555, and Ser777. Conversely, mTOR, which is a regulator of cell growth and is an inhibitor of autophagy, phosphorylates ULK1 at Ser757 and disrupts the interaction between ULK1 and AMPK.
| Purification Method | Affinity purification |
| Gene ID | 8408 9706 |
| RRID | AB_2862420 |
| Buffer Information | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of various lysates using ULK1/ULK2 Rabbit pAb (CAB18685) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 90s. |
