Anti-UMPS Antibody (CAB13251)

Antibody Name:	Anti-UMPS Antibody
Antibody SKU:	CAB13251
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-260 of human UMPS (NP_000364.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, U-87MG, HeLa, LO2, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 100-260 of human UMPS (NP_000364.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TKDY GTKR LVEG TINP GETC LIIE DVVT SGSS VLET VEVL QKEG LKVT DAIV LLDR EQGG KDKL QAHG IRLH SVCT LSKM LEIL EQQK KVDA ETVG RVKR FIQE NVFV AANH NGSP LSIK EAPK ELSF GARA ELPR IHPV ASKL LRLM QKKE TNLC LSAD V
Gene ID:	7372
Uniprot:	P11172
Cellular Location:
Calculated MW:	22kDa/33kDa/42kDa/52kDa
Observed MW:	57kDa

Synonyms:

UMPS, OPRT

Background:

This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	UMPS: Defects in UMPS are the cause of orotic aciduria type 1 (ORAC1). A disorder of pyrimidine metabolism resulting in megaloblastic anemia and orotic acid crystalluria that is frequently associated with some degree of physical and mental retardation. A minority of cases have additional features, particularly congenital malformations and immune deficiencies. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 2.4.2.10; Xenobiotic Metabolism - drug metabolism - other enzymes; Transferase; Lyase; EC 4.1.1.23; Nucleotide Metabolism - pyrimidine Chromosomal Location of Human Ortholog: 3q13 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:orotate phosphoribosyltransferase activity; orotidine-5'-phosphate decarboxylase activity Biological Process: pyrimidine nucleoside biosynthetic process; UMP biosynthetic process Disease: Orotic Aciduria
NCBI Summary:	This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]
UniProt Code:	P11172
NCBI GenInfo Identifier:	131708
NCBI Gene ID:	7372
NCBI Accession:	P11172.1
UniProt Secondary Accession:	P11172,O00758, O00759, O00760, Q16862, Q9H3Q2, Q9UG49 B5LY68, B5LY72,
UniProt Related Accession:	P11172
Molecular Weight:	22,922 Da
NCBI Full Name:	Uridine 5'-monophosphate synthase
NCBI Synonym Full Names:	uridine monophosphate synthetase
NCBI Official Symbol:	UMPS
NCBI Official Synonym Symbols:	OPRT
NCBI Protein Information:	uridine 5'-monophosphate synthase
UniProt Protein Name:	Uridine 5'-monophosphate synthase
UniProt Synonym Protein Names:	Orotate phosphoribosyltransferase (EC:2.4.2.10); OPRT; OPRTaseOrotidine 5'-phosphate decarboxylase (EC:4.1.1.23); ODCAlternative name(s):OMPdecase
Protein Family:	Uridine 5'-monophosphate synthase
UniProt Gene Name:	UMPS
UniProt Entry Name:	UMPS_HUMAN

	Western blot analysis of extracts of various cell lines, using UMPS antibody (CAB13251) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunofluorescence analysis of NIH-3T3 cells using UMPS Polyclonal Antibody (CAB13251) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies

Anti-UMPS Antibody (CAB5492)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)