Anti-UNC13B Antibody (CAB15780)

Antibody Name:	Anti-UNC13B Antibody
Antibody SKU:	CAB15780
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human UNC13B (NP_006368.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	293T, LO2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human UNC13B (NP_006368.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSLL CVRV KRAK FQGS PDKF NTYV TLKV QNVK STTV AVRG DQPS WEQD FMFE ISRL DLGL SVEV WNKG LIWD TMVG TVWI ALKT IRQS DEEG PGEW STLE AETL MKDD EICG TRNP TPHK ILLD TRFE LPFD IPEE EARY WTYK WEQI NALG ADNE YSSQ EESQ RKPL PTAA AQCS FEDP DSAV DDRD SDYR SETS NSFP PPYH TASQ PNAS VHQF PVPV RSPQ QLLL QGSS RDSC NDSM QSYD LDYP ERRA ISPT SSSR YGSS CNVS QGSS QLSE LDQY HEQD DDHR ETDS IHSC HSSH SLSR DGQA GFGE QEKP LEVT GQAE KEAA CEPK EMKE DATT HPPP DLVL QK
Gene ID:	10497
Uniprot:	O14795
Cellular Location:	Cell junction, Cell membrane, Cytoplasm, Membrane, Peripheral membrane protein, synapse
Calculated MW:	180kDa/182kDa
Observed MW:	210kDa

Synonyms:

UNC13B, MUNC13, UNC13, Unc13h2

Background:

This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis.

UniProt Protein Function:	UNC13B: Plays a role in vesicle maturation during exocytosis as a target of the diacylglycerol second messenger pathway. Is involved in neurotransmitter release by acting in synaptic vesicle priming prior to vesicle fusion and participates in the activity- depending refilling of readily releasable vesicle pool (RRP). Essential for synaptic vesicle maturation in a subset of excitatory/glutamatergic but not inhibitory/GABA-mediated synapses. Belongs to the unc-13 family.
UniProt Protein Details:	Protein type:Lipid-binding Chromosomal Location of Human Ortholog: 9p13.3 Cellular Component: Golgi apparatus; cytoplasm; presynaptic active zone; plasma membrane; terminal button; neuromuscular junction; cytosol; cell junction Molecular Function:diacylglycerol binding; protein binding; signal transducer activity; metal ion binding; receptor activity Biological Process: synaptic transmission; synaptic transmission, glutamatergic; glutamate secretion; apoptosis; neurotransmitter secretion; regulation of short-term neuronal synaptic plasticity; excretion; signal transduction; synaptic vesicle priming; neuromuscular junction development; synaptic vesicle docking during exocytosis
NCBI Summary:	This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq, Jul 2008]
UniProt Code:	O14795
NCBI GenInfo Identifier:	2432000
NCBI Gene ID:	10497
NCBI Accession:	AAC19406.1
UniProt Secondary Accession:	O14795,Q5VYM8,
UniProt Related Accession:	O14795
Molecular Weight:	180,679 Da
NCBI Full Name:	Munc13
NCBI Synonym Full Names:	unc-13 homolog B (C. elegans)
NCBI Official Symbol:	UNC13B
NCBI Official Synonym Symbols:	UNC13; MUNC13; Unc13h2
NCBI Protein Information:	protein unc-13 homolog B; munc13-2; homolog of rat Munc13 (diacylglycerol-binding)
UniProt Protein Name:	Protein unc-13 homolog B
UniProt Synonym Protein Names:	Munc13-2
Protein Family:	Protein
UniProt Gene Name:	UNC13B
UniProt Entry Name:	UN13B_HUMAN

	Western blot analysis of extracts of various cell lines, using UNC13B antibody (CAB15780) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded Human liver using UNC13B Rabbit pAb (CAB15780) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of L929 cells using UNC13B antibody (CAB15780) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)