The USP30 Antibody (CAB12862) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards USP30 in human samples, making it ideal for use in Western blot applications.USP30 is a deubiquitinating enzyme located in the outer mitochondrial membrane, where it plays a crucial role in the maintenance of mitochondrial function and integrity. Dysregulation of USP30 has been implicated in various diseases, including neurodegenerative disorders and cancer, highlighting its importance as a therapeutic target.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
USP30 Antibody
SKU:
CAB12862
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
USP30
Positive Sample:
Mouse lung, Rat lung
Cellular Localization:
Mitochondrion Outer Membrane.
Calculated MW:
59kDa
Observed MW:
71kDa
USP30, a member of the ubiquitin-specific protease family (see USP1, MIM 603478), is a novel mitochondrial deubiquitinating (DUB) enzyme (Nakamura and Hirose, 2008 [PubMed 18287522]).
Purification Method
Affinity purification
Gene ID
84749
RRID
AB_2759703
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using USP30 Rabbit pAb (CAB12862) at 1:3000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunofluorescence analysis of NIH-3T3 cells using USP30 Rabbit pAb (CAB12862) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
