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Anti-VAMP3 Antibody (CAB7457)

SKU:
CAB7457
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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Antibody Name:Anti-VAMP3 Antibody
Antibody SKU:CAB7457
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human VAMP3 (NP_004772.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:SKOV3, U-251MG, SW480, A-549, 293T, Mouse testis, Mouse kidney, Mouse lung, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human VAMP3 (NP_004772.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSTG PTAA TGSN RRLQ QTQN QVDE VVDI MRVN VDKV LERD QKLS ELDD RADA LQAG ASQF ETSA AKLK RKYW WKNC KMWA
Gene ID:9341
Uniprot:Q15836
Cellular Location:Cell junction, Membrane, Single-pass type IV membrane protein, synapse, synaptosome
Calculated MW:11kDa
Observed MW:15kDa
Synonyms:VAMP3, CEB
Background:Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation.
UniProt Protein Function:VAMP3: SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network. Belongs to the synaptobrevin family.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p36.23

Cellular Component: neuron projection; cell surface; intracellular membrane-bound organelle; clathrin-coated vesicle; integral to membrane; clathrin coated vesicle membrane; cytosol; secretory granule; SNARE complex; synaptic vesicle; recycling endosome; apical plasma membrane; plasma membrane; intracellular; cell junction

Molecular Function:SNAP receptor activity; SNARE binding; protein binding; syntaxin-1 binding

Biological Process: vesicle fusion; vesicle-mediated transport; exocytosis; neurotransmitter secretion; Golgi to plasma membrane protein transport; protein complex assembly; positive regulation of immunoglobulin secretion; positive regulation of receptor recycling; retrograde transport, endosome to Golgi; vesicle docking during exocytosis; calcium ion-dependent exocytosis

NCBI Summary:Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Because of its high homology to other known VAMPs, its broad tissue distribution, and its subcellular localization, the protein encoded by this gene was shown to be the human equivalent of the rodent cellubrevin. In platelets the protein resides on a compartment that is not mobilized to the plasma membrane on calcium or thrombin stimulation. [provided by RefSeq, Jul 2008]
UniProt Code:Q15836
NCBI GenInfo Identifier:2501082
NCBI Gene ID:9341
NCBI Accession:Q15836.3
UniProt Secondary Accession:Q15836,P63024, P63025,
UniProt Related Accession:Q15836
Molecular Weight:
NCBI Full Name:Vesicle-associated membrane protein 3
NCBI Synonym Full Names:vesicle associated membrane protein 3
NCBI Official Symbol:VAMP3  
NCBI Official Synonym Symbols:CEB  
NCBI Protein Information:vesicle-associated membrane protein 3
UniProt Protein Name:Vesicle-associated membrane protein 3
UniProt Synonym Protein Names:Cellubrevin; CEB; Synaptobrevin-3
UniProt Gene Name:VAMP3  
UniProt Entry Name:VAMP3_HUMAN
Anti-VAMP3 Antibody (CAB7457)Western blot analysis of extracts of various cell lines, using VAMP3 antibody (CAB7457) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 5s.

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