The VGLL4 Antibody (CAB17058) is a high-quality antibody developed for reliable detection and analysis of target proteins. The antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various experimental applications, including Western blot and immunohistochemistry.VGLL4 is known to interact with various transcription factors, influencing gene expression and cellular responses. Its role in regulating key pathways involved in cell proliferation and differentiation makes it a key target for research in fields such as developmental biology, cancer biology, and regenerative medicine.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Mouse, Rat samples.
Product Name:
VGLL4 Antibody
SKU:
CAB17058
Size:
20μL, 100μL
Reactivity:
Mouse, Rat
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VGL-4, VGLL4
Positive Sample:
Mouse spleen, Rat lung, Rat spleen
Cellular Localization:
Nucleus.
Calculated MW:
31kDa
Observed MW:
31kDa
Predicted to enable transcription coactivator binding activity. Involved in negative regulation of Wnt signaling pathway; negative regulation of cell growth; and negative regulation of hippo signaling. Predicted to be located in nucleus.
Purification Method
Affinity purification
Gene ID
9686
RRID
AB_2772856
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using VGLL4 Rabbit pAb (CAB17058) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 60s.
