Anti-VTN Antibody (CAB1667)

Antibody Name:	Anti-VTN Antibody
Antibody SKU:	CAB1667
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 364-478 of human VTN (NP_000629.3).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Human serum, Rat serum

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 364-478 of human VTN (NP_000629.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SLAK KQRF RHRN RKGY RSQR GHSR GRNQ NSRR PSRA TWLS LFSS EESN LGAN NYDD YRMD WLVP ATCE PIQS VFFF SGDK YYRV NLRT RRVD TVDP PYPR SIAQ YWLG CPAP GHL
Gene ID:	7448
Uniprot:	P04004
Cellular Location:	Secreted, extracellular space
Calculated MW:	54kDa
Observed MW:	65kDa, 75kDa

Synonyms:

VTN, V75, VN, VNT

Background:

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

UniProt Protein Function:	VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
UniProt Protein Details:	Protein type:Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Cell adhesion Chromosomal Location of Human Ortholog: 17q11 Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region Molecular Function:heparin binding; integrin binding; protein binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding Biological Process: receptor-mediated endocytosis; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; negative regulation of blood coagulation; positive regulation of smooth muscle cell migration; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of receptor-mediated endocytosis; positive regulation of peptidyl-tyrosine phosphorylation; regulation of complement activation; cell adhesion mediated by integrin; innate immune response; immune response; cell adhesion
NCBI Summary:	The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]
UniProt Code:	P04004
NCBI GenInfo Identifier:	139653
NCBI Gene ID:	7448
NCBI Accession:	P04004.1
UniProt Related Accession:	P04004
Molecular Weight:
NCBI Full Name:	Vitronectin
NCBI Synonym Full Names:	vitronectin
NCBI Official Symbol:	VTN
NCBI Official Synonym Symbols:	VN; V75; VNT
NCBI Protein Information:	vitronectin
UniProt Protein Name:	Vitronectin
UniProt Synonym Protein Names:	S-protein; Serum-spreading factor; V75Cleaved into the following 3 chains:Vitronectin V65 subunit; Vitronectin V10 subunit; Somatomedin-B
Protein Family:	Vitronectin
UniProt Gene Name:	VTN
UniProt Entry Name:	VTNC_HUMAN

	Western blot analysis of extracts of various cell lines, using VTN antibody (CAB1667) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunohistochemistry of paraffin-embedded mouse lung using VTN antibody (CAB1667) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse leydig cells using VTN antibody (CAB1667) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-VTN Antibody (CAB13561)	Human Vitronectin ELISA Kit
Anti-Vitronectin Antibody (CAB4883)	Human VTN (Vitronectin) CLIA Kit (HUES00643)
VTN Antibody (PACO05306)	Human Vitronectin (VTN) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)