The Vitronectin Monoclonal Antibody (CAB4883) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, demonstrates high specificity and sensitivity for detecting vitronectin in various samples, particularly in Western blot applications.Vitronectin plays a crucial role in cell adhesion, migration, and regulation of the coagulation cascade, making it a key player in processes such as wound healing, tissue remodeling, and angiogenesis. Dysregulation of vitronectin expression has been implicated in various diseases, including cancer, cardiovascular disorders, and inflammatory conditions.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
Vitronectin Monoclonal Antibody
SKU:
CAB4883
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC0313
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
VN, V75, VNT, Vitronectin
Positive Sample:
HepG2
Cellular Localization:
Secreted, Extracellular Space.
Calculated MW:
54kDa
Observed MW:
75kDa
The protein encoded by this gene functions in part as an adhesive glycoprotein. Differential expression of this protein can promote either cell adhesion or migration as it links cells to the extracellular matrix through a variety of ligands. These ligands include integrins, plasminogen activator inhibitor-1, and urokinase plasminogen activator receptor. This secreted protein can be present in the plasma as a monomer or dimer and forms a multimer in the extracellular matrix of several tissues. This protein also inhibits the membrane-damaging effect of the terminal cytolytic complement pathway and binds to several serpin serine protease inhibitors. This protein can also promote extracellular matrix degradation and thus plays a role in tumorigenesis. It is involved in a variety of other biological processes such as the regulation of the coagulation pathway, wound healing, and tissue remodeling. The heparin-binding domain of this protein give it anti-microbial properties. It is also a lipid binding protein that forms a principal component of high density lipoprotein.
Purification Method
Affinity purification
Gene ID
7448
RRID
AB_2863372
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from HepG2 cells, using Vitronectin Rabbit mAb (CAB4883) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 3min.
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