The VMA ELISA Kit is designed for the quantitative detection of Vanillylmandelic Acid (VMA) levels in various biological samples. VMA is a metabolite of epinephrine and norepinephrine, and its levels can be used as a biomarker for diseases like neuroblastoma and pheochromocytoma. Accurate measurement of VMA is critical for uncovering its role in disease mechanisms, as well as aiding in diagnostic processes and the development of targeted therapeutic strategies. Assay Genie’s VMA ELISA Kit offers outstanding sensitivity and specificity, ensuring trustworthy and replicable results. Fabricated under stringent quality control standards, this kit offers robust performance, simplicity of use, and is an excellent choice for both research and clinical applications. Trust Assay Genie’s VMA ELISA Kit for accurate and reliable quantification of this important biomarker in your studies.
Product Name:
VMA (Vanillylmandelic Acid) ELISA Kit
SKU:
AEES00009
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
