Anti-VRK1 Antibody (CAB7745)

Antibody Name:	Anti-VRK1 Antibody
Antibody SKU:	CAB7745
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 257-396 of human VRK1 (NP_003375.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF-7, Jurkat, A-549, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 257-396 of human VRK1 (NP_003375.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GHLP WEDN LKDP KYVR DSKI RYRE NIAS LMDK CFPE KNKP GEIA KYME TVKL LDYT EKPL YENL RDIL LQGL KAIG SKDD GKLD LSVV ENGG LKAK TITK KRKK EIEE SKEP GVED TEWS NTQT EEAI QTRS RTRK RVQK
Gene ID:	7443
Uniprot:	Q99986
Cellular Location:	Cytoplasm, Nucleus, cytoskeleton, spindle
Calculated MW:	45kDa
Observed MW:	45kDa

Synonyms:

VRK1, PCH1, PCH1A

Background:

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN.

UniProt Protein Function:	VRK1: Serine/threonine kinase that phosphorylates Thr-18 of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1); also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy (SMA). Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.
UniProt Protein Details:	Protein type:Kinase, protein; EC 2.7.11.1; Protein kinase, CK1; Protein kinase, Ser/Thr (non-receptor); CK1 group; VRK family Chromosomal Location of Human Ortholog: 14q32 Cellular Component: cytoplasm; cytosol; Golgi stack; nucleolus; nucleoplasm; nucleus Molecular Function:histone serine kinase activity (H3-S10 specific); nucleosomal histone binding; protein binding; protein kinase activity; protein kinase binding; protein serine/threonine kinase activity Biological Process: mitotic nuclear envelope disassembly; mitotic nuclear envelope reassembly; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of cell shape Disease: Pontocerebellar Hypoplasia, Type 1a
NCBI Summary:	This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. Its protein localizes to the nucleus and has been shown to promote the stability and nuclear accumulation of a transcriptionally active p53 molecule and, in vitro, to phosphorylate Thr18 of p53 and reduce p53 ubiquitination. This gene, therefore, may regulate cell proliferation. This protein also phosphorylates histone, casein, and the transcription factors ATF2 (activating transcription factor 2) and c-JUN. [provided by RefSeq, Jul 2008]
UniProt Code:	Q99986
NCBI GenInfo Identifier:	45593726
NCBI Gene ID:	7443
NCBI Accession:	Q99986.1
UniProt Secondary Accession:	Q99986,Q3SYL2,
UniProt Related Accession:	Q99986
Molecular Weight:	45,476 Da
NCBI Full Name:	Serine/threonine-protein kinase VRK1
NCBI Synonym Full Names:	vaccinia related kinase 1
NCBI Official Symbol:	VRK1
NCBI Official Synonym Symbols:	PCH1; PCH1A
NCBI Protein Information:	serine/threonine-protein kinase VRK1
UniProt Protein Name:	Serine/threonine-protein kinase VRK1
UniProt Synonym Protein Names:	Vaccinia-related kinase 1
Protein Family:	Serine/threonine-protein kinase
UniProt Gene Name:	VRK1
UniProt Entry Name:	VRK1_HUMAN

	Western blot analysis of extracts of various cell lines, using VRK1 antibody (CAB7745) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat lung using VRK1 antibody (CAB7745) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using VRK1 antibody (CAB7745) at dilution of 1:100 (40x lens).

Antibodies

Anti-VRK1 Antibody (CAB19938)[KO Validated]

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)