Anti-WARS Antibody (CAB5758)

Antibody Name:	Anti-WARS Antibody
Antibody SKU:	CAB5758
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WARS (NP_776049.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF7, SW480, 293T, HeLa, mouse brain, mouse lung, rat brain, HL-60, HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human WARS (NP_776049.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPNS EPAS LLEL FNSI ATQG ELVR SLKA GNAS KDEI DSAV KMLV SLKM SYKA AAGE DYKA DCPP GNPA PTSN HGPD ATEA EEDF VDPW TVQT SSAK GIDY DKLI VRFG SSKI DKEL INRI ERAT GQRP HHFL RRGI FFSH RDMN QVLD AYEN KKPF YLYT GRGP SSEA MHVG HLIP FIFT KWLQ DVFN VPLV IQMT DDEK YLWK DLTL DQAY SYAV ENAK DIIA CGFD INKT FIFS DLDY MGMS SGFY KNVV KIQK HVTF NQVK GIFG FT
Gene ID:	7453
Uniprot:	P23381
Cellular Location:	Cytoplasm
Calculated MW:	48kDa/53kDa
Observed MW:	53-55kDa

Synonyms:

WARS, GAMMA-2, IFI53, IFP53

Background:

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	WARS: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Translation; Amino Acid Metabolism - tryptophan; Ligase; EC 6.1.1.2 Chromosomal Location of Human Ortholog: 14q32.31 Cellular Component: cytoplasm; nucleus; cytosol Molecular Function:protein binding; tryptophan-tRNA ligase activity; ATP binding Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; tryptophanyl-tRNA aminoacylation; translation; gene expression; angiogenesis; regulation of angiogenesis
NCBI Summary:	Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	P23381
NCBI GenInfo Identifier:	135191
NCBI Gene ID:	7453
NCBI Accession:	P23381.2
UniProt Secondary Accession:	P23381,P78535, Q502Y0, Q53XB6, Q9UDI5, Q9UDL3, A6NGN1 A6NID3,
UniProt Related Accession:	P23381
Molecular Weight:	471
NCBI Full Name:	Tryptophan--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:	tryptophanyl-tRNA synthetase
NCBI Official Symbol:	WARS
NCBI Official Synonym Symbols:	IFI53; IFP53; GAMMA-2
NCBI Protein Information:	tryptophan--tRNA ligase, cytoplasmic; hWRS; trpRS; interferon-induced protein 53; tryptophan tRNA ligase 1, cytoplasmic
UniProt Protein Name:	Tryptophan--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:	Interferon-induced protein 53; IFP53; Tryptophanyl-tRNA synthetase; TrpRS; hWRS
Protein Family:	Tryptophan--tRNA ligase
UniProt Gene Name:	WARS
UniProt Entry Name:	SYWC_HUMAN

	Western blot analysis of extracts of various cell lines, using WARS antibody (CAB5758) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunofluorescence analysis of U2OS cells using WARS antibody (CAB5758). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
Anti-TrpRS Antibody (CAB4469)	Human Tryptophanyl tRNA Synthetase / WARS ELISA Kit
	Human Tryptophanyl-tRNA synthetase, cytoplasmic (WARS) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)