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Anti-WDR48 Antibody (CAB6854)

SKU:
CAB6854
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology
  • Immunology Antibodies 2 Anti-WDR48 Antibody CAB6854
  • Immunology Antibodies 2 Anti-WDR48 Antibody CAB6854
  • Immunology Antibodies 2 Anti-WDR48 Antibody CAB6854
  • Immunology Antibodies 2 Anti-WDR48 Antibody CAB6854
€139 - €419
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Description

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Antibody Name:Anti-WDR48 Antibody
Antibody SKU:CAB6854
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 450-677 of human WDR48 (NP_065890.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:U-251MG, A-431, SGC-7901, LO2, A-549, HT-1080, Mouse brain, Rat testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 450-677 of human WDR48 (NP_065890.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:FSSP DGSD PKLN LGGL LLQA LLEY WPRT HVNP MDEE ENEV NHVN GEQE NRVQ KGNG YFQV PPHT PVIF GEAG GRTL FRLL CRDS GGET ESML LNET VPQW VIDI TVDK NMPK FNKI PFYL QPHA SSGA KTLK KDRL SASD MLQV RKVM EHVY EKII NLDN ESQT TSSS NNEK PGEQ EKEE DIAV LAEE KIEL LCQD QVLD PNMD LRTV KHFI WKSG GDLT LHYR QKST
Gene ID:57599
Uniprot:Q8TAF3
Cellular Location:Cytoplasm, Lysosome, Nucleus
Calculated MW:13kDa/45kDa/66kDa/75kDa/76kDa
Observed MW:76kDa
Synonyms:WDR48, P80, SPG60, UAF1
Background:The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage.
UniProt Protein Function:WDR48: an ubiquitous lysosomal WD40 repeat protein. May play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP.
UniProt Protein Details:

Protein type:Ubiquitin conjugating system; Vesicle

Chromosomal Location of Human Ortholog: 3p21.33

Cellular Component: intracellular membrane-bound organelle; lysosome; nucleus

Molecular Function:protein binding

Biological Process: skin development; protein deubiquitination; viral reproduction; skeletal morphogenesis; single fertilization; multicellular organism growth; homeostasis of number of cells; embryonic organ development; spermatogenesis; double-strand break repair via homologous recombination; positive regulation of epithelial cell proliferation

NCBI Summary:The protein encoded by this gene has been shown to interact with ubiquitin specific peptidase 1 (USP1), activating the deubiquitinating activity of USP1 and allowing it to remove the ubiquitin moiety from monoubiquitinated FANCD2. FANCD2 is ubiquitinated in response to DNA damage. [provided by RefSeq, Sep 2016]
UniProt Code:Q8TAF3
NCBI GenInfo Identifier:74760390
NCBI Gene ID:57599
NCBI Accession:Q8TAF3.1
UniProt Related Accession:Q8TAF3
Molecular Weight:76kDa
NCBI Full Name:WD repeat-containing protein 48
NCBI Synonym Full Names:WD repeat domain 48
NCBI Official Symbol:WDR48  
NCBI Official Synonym Symbols:P80; UAF1; SPG60  
NCBI Protein Information:WD repeat-containing protein 48
UniProt Protein Name:WD repeat-containing protein 48
UniProt Synonym Protein Names:USP1-associated factor 1; WD repeat endosomal protein; p80
Protein Family:WD repeat-containing protein
UniProt Gene Name:WDR48  
UniProt Entry Name:WDR48_HUMAN
Anti-WDR48 Antibody (CAB6854)Western blot analysis of extracts of various cell lines, using WDR48 antibody (CAB6854) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
Anti-WDR48 Antibody (CAB6854)Immunohistochemistry of paraffin-embedded rat liver using WDR48 antibody (CAB6854) at dilution of 1:100 (40x lens).
Anti-WDR48 Antibody (CAB6854)Immunohistochemistry of paraffin-embedded human liver using WDR48 antibody (CAB6854) at dilution of 1:100 (40x lens).
Anti-WDR48 Antibody (CAB6854)Immunohistochemistry of paraffin-embedded human stomach using WDR48 antibody (CAB6854) at dilution of 1:100 (40x lens).

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