Anti-WDR61 Antibody (CAB15520)

Antibody Name:	Anti-WDR61 Antibody
Antibody SKU:	CAB15520
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human WDR61 (NP_079510.1).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, A-549, mouse stomach, mouse brain, mouse kidney, rat pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human WDR61 (NP_079510.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MTNQ YGIL FKQE QAHD DAIW SVAW GTNK KENS ETVV TGSL DDLV KVWK WRDE RLDL QWSL EGHQ LGVV SVDI SHTL PIAA SSSL DAHI RLWD LENG KQIK SIDA GPVD AWTL AFSP DSQY LATG THVG KVNI FGVE SGKK EYSL DTRG KFIL SIAY SPDG KYLA SGAI DGII NIFD IATG KLLH TLEG HAMP IRSL TFSP DSQL LVTA SDDG YIKI YDVQ HANL AGTL SGHA SWVL NVAF CPDD THFV SSSS DKSV KVWD VGTR TCVH TFFD HQDQ VWGV KYNG NGSK IVSV GDDQ EIHI YDCP I
Gene ID:	80349
Uniprot:	Q9GZS3
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	33kDa
Observed MW:	36kDa

Synonyms:	WDR61, REC14, SKI8
Background:

UniProt Protein Function:	WDR61: Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non- phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of MLL1; it promotes leukemogenesis though association with MLL-rearranged oncoproteins, such as MLL-MLLT3/AF9 and MLL-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Required for mono- and trimethylation on histone H3 'Lys-4' (H3K4me3), dimethylation on histone H3 'Lys-79' (H3K4me3). Required for Hox gene transcription. Component of the SKI complex which is thought to be involved in exosome-mediated RNA decay and associates with transcriptionally active genes in a manner dependent on PAF1C.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 15q25.1 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:protein binding Biological Process: negative regulation of myeloid cell differentiation; positive regulation of histone H3-K4 methylation; positive regulation of RNA elongation from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter
NCBI Summary:	WDR61 is a subunit of the human PAF and SKI complexes, which function in transcriptional regulation and are involved in events downstream of RNA synthesis, such as RNA surveillance (Zhu et al., 2005 [PubMed 16024656]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q9GZS3
NCBI GenInfo Identifier:	74761365
NCBI Gene ID:	80349
NCBI Accession:	Q9GZS3.1
UniProt Secondary Accession:	Q9GZS3,Q6IA22, Q7Z4X4, D3DW84,
UniProt Related Accession:	Q9GZS3
Molecular Weight:	33,581 Da
NCBI Full Name:	WD repeat-containing protein 61
NCBI Synonym Full Names:	WD repeat domain 61
NCBI Official Symbol:	WDR61
NCBI Official Synonym Symbols:	SKI8; REC14
NCBI Protein Information:	WD repeat-containing protein 61
UniProt Protein Name:	WD repeat-containing protein 61
UniProt Synonym Protein Names:	Meiotic recombination REC14 protein homolog; SKI8 homolog; Ski8
Protein Family:	WD repeat-containing protein
UniProt Gene Name:	WDR61
UniProt Entry Name:	WDR61_HUMAN

	Western blot analysis of extracts of various cell lines, using WDR61 antibody (CAB15520) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 40s.
	Immunohistochemistry of paraffin-embedded Human placenta using WDR61 Rabbit pAb (CAB15520) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded Rat brain using WDR61 Rabbit pAb (CAB15520) at dilution of 1:100 (40x lens).

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)