Anti-WDR77 Antibody (CAB7134)

Antibody Name:	Anti-WDR77 Antibody
Antibody SKU:	CAB7134
Antibody Size:	20uL, 50uL, 100uL
Application:	IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WDR77 (NP_077007.1).

Application:	IHC IF
Recommended Dilution:	IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WDR77 (NP_077007.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRKE TPPP LVPP AARE WNLP PNAP ACME RQLE AARY RSDG ALLL GASS LSGR CWAG SLWL FKDP CAAP NEGF CSAG VQTE AGVA DLTW VGER GILV ASDS GAVE LWEL DENE TLIV SKFC KYEH DDIV STVS VLSS GTQA VSGS KDIC IKVW DLAQ QVVL SSYR AHAA QVTC VAAS PHKD SVFL SCSE DNRI LLWD TRCP KPAS QIGC SAPG YLPT SLAW HPQQ SEVF VFGD ENGT VSLV DTKS TSCV LSSA VHSQ CVTG
Gene ID:	79084
Uniprot:	Q9BQA1
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	29kDa/36kDa
Observed MW:	Refer to figures

Synonyms:

WDR77, HKMT1069, MEP-50, MEP50, Nbla10071, p44, p44/Mep50

Background:

The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	WDR77: a WD40 repeat protein. Component of the methylosome, a 20S complex containing at least ICLN, SKB1 and WDR77. Interacts with SKB1 and with SM proteins. The methylosome may regulate an early step in the assembly of U snRNPs, possibly the transfer of Sm proteins to the SMN-complex. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.
UniProt Protein Details:	Protein type:Transcription, coactivator/corepressor; Adaptor/scaffold; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 1p13.2 Cellular Component: nucleoplasm; Golgi apparatus; cytoplasm; cytosol; nucleus Molecular Function:protein binding; ligand-dependent nuclear receptor transcription coactivator activity Biological Process: regulation of transcription from RNA polymerase II promoter; spliceosomal snRNP biogenesis; establishment and/or maintenance of chromatin architecture; positive regulation of cell proliferation; gene expression
NCBI Summary:	WDR77 is a component of the 20S PRMT5 (MIM 604045)-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (see MIM 601061). This modification targets Sm proteins to the survival of motor neurons (SMN) complex (see MIM 600354) for assembly into small nuclear ribonucleoprotein core particles (Friesen et al., 2002 [PubMed 11756452]).[supplied by OMIM, Mar 2008]
UniProt Code:	Q9BQA1
NCBI GenInfo Identifier:	32171507
NCBI Gene ID:	79084
NCBI Accession:	Q9BQA1.1
UniProt Secondary Accession:	Q9BQA1,Q3LID2, Q53FU2, Q6JZZ5, Q96GK4, Q9BWY3, B3KMW6 B4DP38,
UniProt Related Accession:	Q9BQA1
Molecular Weight:	342
NCBI Full Name:	Methylosome protein 50
NCBI Synonym Full Names:	WD repeat domain 77
NCBI Official Symbol:	WDR77
NCBI Official Synonym Symbols:	p44; MEP50; MEP-50; HKMT1069; Nbla10071; p44/Mep50
NCBI Protein Information:	methylosome protein 50; androgen receptor cofactor p44; WD repeat-containing protein 77
UniProt Protein Name:	Methylosome protein 50
UniProt Synonym Protein Names:	Androgen receptor cofactor p44; WD repeat-containing protein 77; p44/Mep50
Protein Family:	Methylosome protein
UniProt Gene Name:	WDR77
UniProt Entry Name:	MEP50_HUMAN

	Immunohistochemistry of paraffin-embedded human prostate using WDR77 antibody (CAB7134) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using WDR77 antibody (CAB7134) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of A-549 cells using WDR77 antibody (CAB7134). Blue: DAPI for nuclear staining.

Antibodies

Anti-WDR77 Monoclonal Antibody (CAB9921)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)