Anti-WISP2 Antibody (CAB7456)

Antibody Name:	Anti-WISP2 Antibody
Antibody SKU:	CAB7456
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human WISP2 (NP_003872.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse lung, Mouse ovary

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human WISP2 (NP_003872.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MRGT PKTH LLAF SLLC LLSK VRTQ LCPT PCTC PWPP PRCP LGVP LVLD GCGC CRVC ARRL GEPC DQLH VCDA SQGL VCQP GAGP GGRG ALCL LAED DSSC EVNG RLYR EGET FQPH CSIR CRCE DGGF TCVP LCSE DVRL PSWD CPHP RRVE VLGK CCPE WVCG QGGG LGTQ PLPA QGPQ FSGL VSSL PPGV PCPE WSTA WGPC STTC GLGM ATRV SNQN RFCR LETQ RRLC LSRP CPPS RGRS PQNS AF
Gene ID:	8839
Uniprot:	O76076
Cellular Location:	Secreted
Calculated MW:	22kDa/26kDa
Observed MW:	27kDa

Synonyms:

WISP2, CCN5, CT58, CTGF-L

Background:

This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.

UniProt Protein Function:	WISP2: May play an important role in modulating bone turnover. Promotes the adhesion of osteoblast cells and inhibits the binding of fibrinogen to integrin receptors. In addition, inhibits osteocalcin production. Belongs to the CCN family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 20q13.12 Cellular Component: extracellular space; proteinaceous extracellular matrix; plasma membrane Molecular Function:heparin binding; integrin binding; insulin-like growth factor binding Biological Process: cell-cell signaling; regulation of cell growth; signal transduction; cell adhesion
NCBI Summary:	This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like (CT) domain. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover. [provided by RefSeq, Jul 2008]
UniProt Code:	O76076
NCBI GenInfo Identifier:	24211561
NCBI Gene ID:	8839
NCBI Accession:	O76076.1
UniProt Secondary Accession:	O76076,Q6PEG3, B2R9N4, E1P612,
UniProt Related Accession:	O76076
Molecular Weight:	Observed: 27kDaCalculated: 22kDa/26kDa
NCBI Full Name:	WNT1-inducible-signaling pathway protein 2
NCBI Synonym Full Names:	WNT1 inducible signaling pathway protein 2
NCBI Official Symbol:	WISP2
NCBI Official Synonym Symbols:	CCN5; CT58; CTGF-L
NCBI Protein Information:	WNT1-inducible-signaling pathway protein 2; WISP-2; CCN family member 5; wnt-1 signaling pathway protein 2; connective tissue growth factor-like protein; connective tissue growth factor-related protein 58
UniProt Protein Name:	WNT1-inducible-signaling pathway protein 2
UniProt Synonym Protein Names:	CCN family member 5; Connective tissue growth factor-like protein; CTGF-L; Connective tissue growth factor-related protein 58
Protein Family:	WNT1-inducible-signaling pathway protein
UniProt Gene Name:	WISP2
UniProt Entry Name:	WISP2_HUMAN

	Western blot analysis of extracts of various cell lines, using WISP2 antibody (CAB7456) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat testis using WISP2 Rabbit pAb (CAB7456) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon using WISP2 Rabbit pAb (CAB7456) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-WISP2 Antibody (CAB12541)	HumanÂ CCN5 (CCNÂ familyÂ memberÂ 5)Â ELISAÂ Kit (HUFI03415)
	Human WNT1-inducible-signaling pathway protein 2 (WISP2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)