Anti-WWP1 Antibody (CAB5269)

Antibody Name:	Anti-WWP1 Antibody
Antibody SKU:	CAB5269
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WWP1 (NP_008944.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	PC-3

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human WWP1 (NP_008944.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MATA SPRS DTSN NHSG RLQL QVTV SSAK LKRK KNWF GTAI YTEV VVDG EITK TAKS SSSS NPKW DEQL TVNV TPQT TLEF QVWS HRTL KADA LLGK ATID LKQA LLIH NRKL ERVK EQLK LSLE NKNG IAQT GELT VVLD GLVI EQEN ITNC SSSP TIEI QENG DALH ENGE PSAR TTAR LAVE GTNG IDNH VPTS TLVQ NSCC SYVV NGDN TPSS PSQV AARP KNTP APKP LASE PADD TVNG ESSS FAPT DNAS VTGT
Gene ID:	11059
Uniprot:	Q9H0M0
Cellular Location:	Cell membrane, Cytoplasm, Nucleus, Peripheral membrane protein
Calculated MW:	13kDa/81kDa/91kDa/105kDa
Observed MW:	95kDa

Synonyms:

WWP1, AIP5, Tiul1, hSDRP1

Background:

WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined.

UniProt Protein Function:	WWP1: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 6.3.2.-; Ligase; EC 6.3.2.19; Ubiquitin conjugating system; Ubiquitin ligase Chromosomal Location of Human Ortholog: 8q21 Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol; ubiquitin ligase complex Molecular Function:protein binding; ubiquitin-protein ligase activity; ligase activity Biological Process: proteasomal ubiquitin-dependent protein catabolic process; entry of virus into host cell; central nervous system development; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination; signal transduction; negative regulation of transcription, DNA-dependent; transmembrane transport
NCBI Summary:	WW domain-containing proteins are found in all eukaryotes and play an important role in the regulation of a wide variety of cellular functions such as protein degradation, transcription, and RNA splicing. This gene encodes a protein which contains 4 tandem WW domains and a HECT (homologous to the E6-associated protein carboxyl terminus) domain. The encoded protein belongs to a family of NEDD4-like proteins, which are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. Alternative splicing of this gene generates at least 6 transcript variants; however, the full length nature of these transcripts has not been defined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9H0M0
NCBI GenInfo Identifier:	32171908
NCBI Gene ID:	11059
NCBI Accession:	Q9H0M0.1
UniProt Secondary Accession:	Q9H0M0,O00307, Q5YLC1, Q96BP4,
UniProt Related Accession:	Q9H0M0
Molecular Weight:	922
NCBI Full Name:	NEDD4-like E3 ubiquitin-protein ligase WWP1
NCBI Synonym Full Names:	WW domain containing E3 ubiquitin protein ligase 1
NCBI Official Symbol:	WWP1
NCBI Official Synonym Symbols:	AIP5; Tiul1; hSDRP1
NCBI Protein Information:	NEDD4-like E3 ubiquitin-protein ligase WWP1; WW domain-containing protein 1; atrophin-1 interacting protein 5; atrophin-1-interacting protein 5; TGIF-interacting ubiquitin ligase 1; Nedd-4-like ubiquitin-protein ligase
UniProt Protein Name:	NEDD4-like E3 ubiquitin-protein ligase WWP1
UniProt Synonym Protein Names:	Atrophin-1-interacting protein 5; AIP5; TGIF-interacting ubiquitin ligase 1; Tiul1; WW domain-containing protein 1
Protein Family:	NEDD4-like E3 ubiquitin-protein ligase
UniProt Gene Name:	WWP1
UniProt Entry Name:	WWP1_HUMAN

Western blot analysis of extracts of PC-3 cells, using WWP1 antibody (CAB5269). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)