The Xanthine Oxidase (XDH) Antibody (CAB13052) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for use in various research applications such as Western blotting.XDH, also known as xanthine dehydrogenase, plays a crucial role in the conversion of hypoxanthine to xanthine and xanthine to uric acid, a process essential for maintaining proper purine levels in the body. Additionally, XDH is involved in the generation of reactive oxygen species and reactive nitrogen species, leading to its implications in oxidative stress and inflammatory responses.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
Xanthine Oxidase (XDH) Antibody
SKU:
CAB13052
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ETST NTVP NTSP TAAS VSA
Tested Applications:
WBELISA
Recommended Dilution:
WB
1:500 - 1:1000
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
XO, XOR, XAN1, Xanthine Oxidase (XDH)
Positive Sample:
LO2, Mouse liver, Rat lung, Rat liver
Cellular Localization:
Cytoplasm, Peroxisome, Secreted.
Calculated MW:
146kDa
Observed MW:
150kDa
Xanthine dehydrogenase belongs to the group of molybdenum-containing hydroxylases involved in the oxidative metabolism of purines. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Xanthine dehydrogenase can be converted to xanthine oxidase by reversible sulfhydryl oxidation or by irreversible proteolytic modification. Defects in xanthine dehydrogenase cause xanthinuria, may contribute to adult respiratory stress syndrome, and may potentiate influenza infection through an oxygen metabolite-dependent mechanism.
Purification Method
Affinity purification
Gene ID
7498
RRID
AB_2759900
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using Xanthine Oxidase (XDH) Rabbit pAb (CAB13052) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Western blot analysis of various lysates using Xanthine Oxidase (XDH) Rabbit pAb (CAB13052) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
