Anti-XPC Antibody (CAB8354)

Antibody Name:	Anti-XPC Antibody
Antibody SKU:	CAB8354
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 700-940 of human XPC (NP_004619.3).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human
Positive Samples:	LO2, HeLa, 293T, HT-1080, Jurkat

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 700-940 of human XPC (NP_004619.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GEVP YKMV KGFS NRAR KARL AEPQ LREE NDLG LFGY WQTE EYQP PVAV DGKV PRNE FGNV YLFL PSMM PIGC VQLN LPNL HRVA RKLD IDCV QAIT GFDF HGGY SHPV TDGY IVCE EFKD VLLT AWEN EQAV IERK EKEK KEKR ALGN WKLL AKGL LIRE RLKR RYGP KSEA AAPH TDAG GGLS SDEE EGTS SQAE AARI LAAS WPQN REDE EKQK LKGG PKKT KREK KAAA SHLF PFEQ L
Gene ID:	7508
Uniprot:	Q01831
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	15kDa/101kDa/105kDa
Observed MW:	130kDa

Synonyms:

XPC, RAD4, XP3, XPCC, p125

Background:

This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including Xeroderma pigmentosum (XP) A-G and V, Cockayne syndrome (CS) A and B, and trichothiodystrophy (TTD) group A, etc. This component, XPC, plays an important role in the early steps of global genome NER, especially in damage recognition, open complex formation, and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene.

UniProt Protein Function:	Involved in global genome nucleotide excision repair (GG-NER) by acting as damage sensing and DNA-binding factor component of the XPC complex. Has only a low DNA repair activity by itself which is stimulated by RAD23B and RAD23A. Has a preference to bind DNA containing a short single-stranded segment but not to damaged oligonucleotides. This feature is proposed to be related to a dynamic sensor Function: XPC can rapidly screen duplex DNA for non-hydrogen-bonded bases by forming a transient nucleoprotein intermediate complex which matures into a stable recognition complex through an intrinsic single-stranded DNA-binding activity.
NCBI Summary:	This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including Xeroderma pigmentosum (XP) A-G and V, Cockayne syndrome (CS) A and B, and trichothiodystrophy (TTD) group A, etc. This component, XPC, plays an important role in the early steps of global genome NER, especially in damage recognition, open complex formation, and repair protein complex formation. Mutations in this gene or some other NER components result in Xeroderma pigmentosum, a rare autosomal recessive disorder characterized by increased sensitivity to sunlight with the development of carcinomas at an early age. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
UniProt Code:	Q01831
NCBI GenInfo Identifier:	224809295
NCBI Gene ID:	7508
NCBI Accession:	NP_004619.3
UniProt Secondary Accession:	Q01831,Q53GT7, Q96AX0, B4DIP3, E9PB96, E9PH69,
UniProt Related Accession:	Q01831
Molecular Weight:	15,712 Da
NCBI Full Name:	DNA repair protein complementing XP-C cells
NCBI Synonym Full Names:	XPC complex subunit, DNA damage recognition and repair factor
NCBI Official Symbol:	XPC
NCBI Official Synonym Symbols:	XP3; RAD4; XPCC; p125
NCBI Protein Information:	DNA repair protein complementing XP-C cells
UniProt Protein Name:	DNA repair protein complementing XP-C cells
UniProt Synonym Protein Names:	Xeroderma pigmentosum group C-complementing protein; p125
Protein Family:	DNA repair protein
UniProt Gene Name:	XPC

	Western blot analysis of extracts of various cell lines, using XPC antibody (CAB8354) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded human liver cancer using XPC antibody (CAB8354) at dilution of 1:100 (40x lens).
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug XPC antibody ( A8354). Western blot was performed from the immunoprecipitate using XPC antibody ( A8354) at a dilition of 1:1000.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)