The YARS Antibody (CAB6791) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By selectively binding to the YARS protein, this antibody enables researchers to detect and analyze YARS expression in a variety of cell types, making it an ideal choice for studies in molecular biology and biochemistry.YARS, also known as tyrosyl-tRNA synthetase, is essential for the accurate translation of genetic information into functional proteins.
This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
YARS Antibody
SKU:
CAB6791
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
YRS, YTS, YARS, TYRRS, CMTDIC, IMNEPD2
Positive Sample:
HeLa, SKOV3, Mouse brain, Mouse skeletal muscle, Rat brain
Cellular Localization:
Cytoplasm.
Calculated MW:
59kDa
Observed MW:
59kDa
Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine.
Purification Method
Affinity purification
Gene ID
8565
RRID
AB_2767374
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using YARS Rabbit pAb (CAB6791) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 1 μg YARS antibody (CAB6791). Western blot was performed from the immunoprecipitate using YARS antibody (CAB6791) at a dilution of 1:1000.
