Anti-YEATS4 Antibody (CAB6318)

Antibody Name:	Anti-YEATS4 Antibody
Antibody SKU:	CAB6318
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human YEATS4 (NP_006521.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human YEATS4 (NP_006521.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MFKR MAEF GPDS GGRV KGVT IVKP IVYG NVAR YFGK KREE DGHT HQWT VYVK PYRN EDMS AYVK KIQF KLHE SYGN PLRV VTKP PYEI TETG WGEF EIII KIFF IDPN ERPV TLYH LLKL FQSD TNAM LGKK TVVS EFYD EMIF QDPT AMMQ QLLT TSRQ LTLG AYKH ETEF AELE VKTR EKLE AAKK KTSF EIAE LKER LKAS RETI NCLK NEIR KLEE DDQA KDI
Gene ID:	8089
Uniprot:	O95619
Cellular Location:	Nucleus
Calculated MW:	26kDa
Observed MW:	27KDa

Synonyms:

YEATS4, 4930573H17Rik, B230215M10Rik, GAS41, NUBI-1, YAF9

Background:

The protein encoded by this gene is found in the nucleoli. It has high sequence homology to human MLLT1, and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 proteins belong to a class of transcription factors, indicating that the encoded protein might also represent a transcription factor. This protein is thought to be required for RNA transcription. This gene has been shown to be amplified in tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.

UniProt Protein Function:	YEATS4: Component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.
UniProt Protein Details:	Protein type:Transcription factor Chromosomal Location of Human Ortholog: 12q13-q15 Cellular Component: nucleoplasm; NuA4 histone acetyltransferase complex; nuclear matrix; cytoplasm; nucleolus Molecular Function:protein C-terminus binding; protein binding; DNA binding; structural constituent of cytoskeleton; transcription factor activity Biological Process: mitosis; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; regulation of growth; positive regulation of transcription, DNA-dependent
NCBI Summary:	The protein encoded by this gene is found in the nucleoli. It has high sequence homology to human MLLT1, and yeast and human MLLT3 proteins. Both MLLT1 and MLLT3 proteins belong to a class of transcription factors, indicating that the encoded protein might also represent a transcription factor. This protein is thought to be required for RNA transcription. This gene has been shown to be amplified in tumors. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
UniProt Code:	O95619
NCBI GenInfo Identifier:	59799075
NCBI Gene ID:	8089
NCBI Accession:	O95619.1
UniProt Secondary Accession:	O95619,Q9NQD0,
UniProt Related Accession:	O95619
Molecular Weight:	227
NCBI Full Name:	YEATS domain-containing protein 4
NCBI Synonym Full Names:	YEATS domain containing 4
NCBI Official Symbol:	YEATS4
NCBI Official Synonym Symbols:	YAF9; GAS41; NUBI-1; 4930573H17Rik; B230215M10Rik
NCBI Protein Information:	YEATS domain-containing protein 4; nuBI1; NuMA binding protein 1; nuMA-binding protein 1; glioma-amplified sequence 41; glioma-amplified sequence-41
UniProt Protein Name:	YEATS domain-containing protein 4
UniProt Synonym Protein Names:	Glioma-amplified sequence 41; Gas41; NuMA-binding protein 1; NuBI-1; NuBI1
Protein Family:	YEATS domain-containing protein
UniProt Gene Name:	YEATS4
UniProt Entry Name:	YETS4_HUMAN

	Western blot analysis of extracts of 293T cells, using YEATS4 antibody (CAB6318) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Immunohistochemistry of paraffin-embedded rat lung using YEATS4 Rabbit pAb (CAB6318) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using YEATS4 Rabbit pAb (CAB6318) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using YEATS4 Rabbit pAb (CAB6318) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
YEATS4 Antibody (PACO05409)	GAS41 Colorimetric Cell-Based ELISA

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)