Western blot analysis of various lysates, using YOD1 antibody (CAB23071) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
The YOD1 Monoclonal Antibody (CAB23071) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, developed using advanced monoclonal antibody technology, specifically targets YOD1 protein in human samples and is optimized for use in Western blotting and immunofluorescence applications.YOD1 is known for its function in mediating protein degradation and regulating cellular pathways related to stress response, inflammation, and DNA damage repair. Research on YOD1 has shown its potential implications in diseases such as cancer, neurodegenerative disorders, and viral infections.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human samples.
Product Name:
YOD1 Monoclonal Antibody
SKU:
CAB23071
Size:
20μL, 100μL
Reactivity:
Human
Clone Number:
ARC60044
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
DUBA8, OTUD2, PRO0907, YOD1
Positive Sample:
K-562, HEL
Cellular Localization:
Cytoplasm, Cytosol.
Calculated MW:
38kDa
Observed MW:
38kDa
Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. The protein encoded by this gene belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain. Alternative splicing results in multiple transcript variants.
Purification Method
Affinity purification
Gene ID
55432
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates, using YOD1 Rabbit mAb (CAB23071) at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 30s.
