Anti-YTHDC2 Antibody (CAB15004)

Antibody Name:	Anti-YTHDC2 Antibody
Antibody SKU:	CAB15004
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1131-1430 of human YTHDC2 (NP_073739.3).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, Mouse testis, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1131-1430 of human YTHDC2 (NP_073739.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	HSLF LRRM RAPS KPWS QVDE ATIR AIIA VLST EEQS AGLQ QPSG IGQR PRPM SSEE LPLA SSWR SNNS RKSS ADTE FSDE CTTA ERVL MKSP SPAL HPPQ KYKD RGIL HPKR GTED RSDQ SSLK STDS SSYP SPCA SPSP PSSG KGSK SPSP RPNM PVRY FIMK SSNL RNLE ISQQ KGIW STTP SNER KLNR AFWE SSIV YLVF SVQG SGHF QGFS RMSS EIGR EKSQ DWGS AGLG GVFK VEWI RKES LPFQ FAHH LLNP WNDN KKVQ ISRD GQEL EPLV GEQL LQLW ERLP LGEK NTTD
Gene ID:	64848
Uniprot:	Q9H6S0
Cellular Location:
Calculated MW:	160kDa
Observed MW:	160KDa

Synonyms:

YTHDC2, CAHL

Background:

This gene encodes a member of the DEAH (Asp-Glu-Ala-His) subfamily of proteins, part of the DEAD (Asp-Glu-Ala-Asp) box family of RNA helicases. The encoded protein binds to N6-methyladenosine, a common modified RNA nucleotide that is enriched in the stop codons and 3' UTRs of eukaryotic messenger RNAs. Binding of proteins to this modified nucleotide may regulate mRNA translation and stability. This gene may be associated with susceptibility to pancreatic cancer in human patients, and knockdown of this gene resulted in reduced proliferation in a human liver cancer cell line. [provided by RefSeq, Sep 2016]

UniProt Protein Function:	YTHDC2: Belongs to the DEAD box helicase family. DEAH subfamily.
UniProt Protein Details:	Protein type:RNA-binding; EC 3.6.4.13 Chromosomal Location of Human Ortholog: 5q22.2 Cellular Component: endoplasmic reticulum; nucleus Molecular Function:protein binding; RNA-dependent ATPase activity; ATP binding; ATP-dependent RNA helicase activity Biological Process: RNA processing
UniProt Code:	Q9H6S0
NCBI GenInfo Identifier:	269847874
NCBI Gene ID:	64848
NCBI Accession:	NP_073739.3
UniProt Secondary Accession:	Q9H6S0,B2RP66,
UniProt Related Accession:	Q9H6S0
Molecular Weight:	160,248 Da
NCBI Full Name:	probable ATP-dependent RNA helicase YTHDC2
NCBI Synonym Full Names:	YTH domain containing 2
NCBI Official Symbol:	YTHDC2
NCBI Official Synonym Symbols:	CAHL
NCBI Protein Information:	probable ATP-dependent RNA helicase YTHDC2; CsA-associated helicase-like protein
UniProt Protein Name:	Probable ATP-dependent RNA helicase YTHDC2
Protein Family:	Probable ATP-dependent RNA helicase
UniProt Gene Name:	YTHDC2
UniProt Entry Name:	YTDC2_HUMAN

	Western blot analysis of extracts of various cell lines, using YTHDC2 Rabbit pAb (CAB15004) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunofluorescence analysis of NIH/3T3 cells using YTHDC2 antibody (CAB15004) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)