Anti-ZBED1 Antibody (CAB6792)

Antibody Name:	Anti-ZBED1 Antibody
Antibody SKU:	CAB6792
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZBED1 (NP_001164607.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human
Positive Samples:	HeLa, BT-474

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ZBED1 (NP_001164607.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MENK SLES SQTD LKLV AHPR AKSK VWKY FGFD TNAE GCIL QWKK IYCR ICMA QIAY SGNT SNLS YHLE KNHP EEFC EFVK SNTE QMRE AFAT AFSK LKPE SSQQ PGQD ALAV KAGH GYDS KKQQ ELTA AVLG LICE GLYP ASIV DEPT FKVL LKTA DPRY ELPS RKYI STKA IPEK YGAV REVI LKEL AEAT WCGI STDM WRSE NQNR AYVT LAAH FLGL GAPN CLSM GSRC LKTF EVPE ENTA ETIT RVLY EVFI EWGI SAKV FGAT TNYG KDIV KACS
Gene ID:	9189
Uniprot:	O96006
Cellular Location:	Nucleus
Calculated MW:	78kDa
Observed MW:	108kDa

Synonyms:

ZBED1, ALTE, DREF, TRAMP, hDREF

Background:

This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.

UniProt Protein Function:	ZBED1: Binds to 5'-TGTCG[CT]GA[CT]A-3' DNA elements found in the promoter regions of a number of genes related to cell proliferation. Binds to the histone H1 promoter and stimulates transcription. Was first identified as gene weakly similar to Ac transposable elements, but does not code for any transposase activity.
UniProt Protein Details:	Protein type:Transcription factor Chromosomal Location of Human Ortholog: Xp22.33;Yp11 Cellular Component: nuclear chromosome Molecular Function:protein dimerization activity; DNA binding; metal ion binding; transposase activity Biological Process: metabolic process
NCBI Summary:	This gene is located in the pseudoautosomal region 1 (PAR1) of X and Y chromosomes. It was earlier identified as a gene with similarity to Ac transposable elements, however, was found not to have transposase activity. Later studies show that this gene product is localized in the nucleus and functions as a transcription factor. It binds to DNA elements found in the promoter regions of several genes related to cell proliferation, such as histone H1, hence may have a role in regulating genes related to cell proliferation. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene. [provided by RefSeq, Jan 2010]
UniProt Code:	O96006
NCBI GenInfo Identifier:	41018380
NCBI Gene ID:	9189
NCBI Accession:	O96006.1
UniProt Secondary Accession:	O96006,Q96BY4,
UniProt Related Accession:	O96006
Molecular Weight:	694
NCBI Full Name:	Zinc finger BED domain-containing protein 1
NCBI Synonym Full Names:	zinc finger, BED-type containing 1
NCBI Official Symbol:	ZBED1
NCBI Official Synonym Symbols:	ALTE; DREF; TRAMP; hDREF
NCBI Protein Information:	zinc finger BED domain-containing protein 1; dREF homolog; Ac-like transposable element; DNA replication-related element binding factor; BED-type zinc finger domain-containing protein 1
UniProt Protein Name:	Zinc finger BED domain-containing protein 1
UniProt Synonym Protein Names:	Putative Ac-like transposable element; dREF homolog
Protein Family:	Zinc finger BED domain-containing protein
UniProt Gene Name:	ZBED1
UniProt Entry Name:	ZBED1_HUMAN

	Western blot analysis of extracts of various cell lines, using ZBED1 Antibody (CAB6792) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunofluorescence analysis of U-2 OS cells using ZBED1 antibody (CAB6792) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)