Anti-ZBTB17 Antibody (CAB8463)

Antibody Name:	Anti-ZBTB17 Antibody
Antibody SKU:	CAB8463
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 121-360 of human ZBTB17 (NP_003434.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:100 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	B cells, U-87MG, Hela

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 121-360 of human ZBTB17 (NP_003434.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	PGGN AEAL ATEG GDKR AKEE KVAT STLS RLEQ AGRS TPIG PSRD LKEE RGGQ AQSA ASGA EQTE KADA PREP PPVE LKPD PTSG MAAA EAEA ALSE SSEQ EMEV EPAR KGEE EQKE QEEQ EEEG AGPA EVKE EGSQ LENG EAPE ENEN EESA GTDS GQEL GSEA RGLR SGTY GDRT ESKA YGSV IHKC EDCG KEFT HTGN FKRH IRIH TGEK PFSC RECS KAFS DPAA CKAH EKTH SPLK
Gene ID:	7709
Uniprot:	Q13105
Cellular Location:	Nucleus
Calculated MW:	79kDa/87kDa/88kDa
Observed MW:	103kDa

Synonyms:	ZBTB17, MIZ-1, ZNF151, ZNF60, pHZ-67
Background:	This gene encodes a zinc finger protein involved in the regulation of c-myc. The symbol MIZ1 has also been associated with PIAS2 which is a different gene located on chromosome 18.

UniProt Protein Function:	ZBTB17: a transcription factor that can function as an activator or repressor depending on its binding partners, and by targeting negative regulators of cell cycle progression. Plays a critical role in early lymphocyte development, where it is essential to prevent apoptosis in lymphoid precursors, allowing them to survive in response to IL7 and undergo proper lineage commitment. Has been shown to bind to the promoters of adenovirus major late protein and cyclin D1 and activate transcription. Required for early embryonic development during gastrulation. Represses RB1 transcription; this repression can be blocked by interaction with ZBTB49 isoform 3/ZNF509S1. Belongs to the krueppel C2H2-type zinc-finger protein family. Binds to the C-terminal helix-loop-helix motif of MYC which inhibits ZBTB17 transactivation and growth arrest activities and renders it insoluble in the nucleus. Also interacts with HCFC1, MAGEA4 and TMPRSS11A. Three isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding; C2H2-type zinc finger protein Chromosomal Location of Human Ortholog: 1p36.13 Cellular Component: nucleoplasm Molecular Function:protein binding; transcription factor activity Biological Process: negative regulation of cell cycle
NCBI Summary:	This gene encodes a zinc finger protein involved in the regulation of c-myc. The symbol MIZ1 has also been associated with PIAS2 which is a different gene located on chromosome 18. [provided by RefSeq, Jul 2008]
UniProt Code:	Q13105
NCBI GenInfo Identifier:	62906906
NCBI Gene ID:	7709
NCBI Accession:	Q13105.3
UniProt Secondary Accession:	Q13105,Q15932, Q5JYB2, Q9NUC9, A0AV07, B4DXB4, B7ZLQ9 F5H411,
UniProt Related Accession:	Q13105
Molecular Weight:	79,228 Da
NCBI Full Name:	Zinc finger and BTB domain-containing protein 17
NCBI Synonym Full Names:	zinc finger and BTB domain containing 17
NCBI Official Symbol:	ZBTB17
NCBI Official Synonym Symbols:	MIZ-1; ZNF60; ZNF151; pHZ-67
NCBI Protein Information:	zinc finger and BTB domain-containing protein 17
UniProt Protein Name:	Zinc finger and BTB domain-containing protein 17
UniProt Synonym Protein Names:	Myc-interacting zinc finger protein 1; Miz-1; Zinc finger protein 151; Zinc finger protein 60
Protein Family:	Zinc finger and BTB domain-containing protein
UniProt Gene Name:	ZBTB17
UniProt Entry Name:	ZBT17_HUMAN

	Western blot analysis of extracts of various cell lines, using ZBTB17 Antibody (CAB8463) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded human liver damage using ZBTB17 antibody (CAB8463) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using ZBTB17 antibody (CAB8463) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using ZBTB17 antibody (CAB8463) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse heart using ZBTB17 antibody (CAB8463) at dilution of 1:100 (40x lens).

Antibodies

Anti-ZBTB17 Antibody (CAB14800)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)