Anti-ZCCHC11 Antibody (CAB5972)

Antibody Name:	Anti-ZCCHC11 Antibody
Antibody SKU:	CAB5972
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human ZCCHC11 (NP_001009881.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:1000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human ZCCHC11 (NP_001009881.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MEES KTLK SENH EPKK NVIC EESK AVQV IGNQ TLKA RNDK SVKE IENS SPNR NSSK KNKQ NDIC IEKT EVKS CKVN AANL PGPK DLGL VLRD QSHC KAKK FPNS PVKA EKAT ISQA KSEK ATSL QAKA EKSP KSPN SVKA EKAS SYQM KSEK VPSS PAEA EKGP SLLL KDMR QKTE LQQI GKKI PSSF TSVD KVNI EAVG GEKC ALQN SPRS QKQQ TCTD NTGD SDDS ASGI EDVS DDLS KMKN DESN KENS SEMD YLEN ATVI DESA LTPE QRLG LKQA EERL ERDH IF
Gene ID:	23318
Uniprot:	Q5TAX3
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	81kDa/185kDa
Observed MW:	185kDa

Synonyms:	ZCCHC11, PAPD3, TUT4
Background:

UniProt Protein Function:	ZCCHC11: a zinc finger protein that may participate in toll-receptor signaling by suppressing TRAF6-dependent activation of NF-kappaB. Two alternatively spliced isoforms have been reported.
UniProt Protein Details:	Protein type:EC 2.7.7.52; RNA processing; Transferase; Cell development/differentiation Chromosomal Location of Human Ortholog: 1p32.3 Cellular Component: extracellular space; cytoplasm; nucleolus Molecular Function:protein binding; zinc ion binding; RNA uridylyltransferase activity Biological Process: inhibition of NF-kappaB transcription factor; pre-microRNA processing; stem cell maintenance; cytokine production; positive regulation of interleukin-6 production; regulation of lipopolysaccharide-mediated signaling pathway; RNA 3'-end processing
NCBI Summary:	ZCCHC11 is an RNA uridyltransferase (EC 2.7.7.52) that uses UTP to add uridines to the 3-prime end of substrate RNA molecules (Jones et al., 2009 [PubMed 19701194]).[supplied by OMIM, Jan 2011]
UniProt Code:	Q5TAX3
NCBI GenInfo Identifier:	116242850
NCBI Gene ID:	23318
NCBI Accession:	Q5TAX3.3
UniProt Secondary Accession:	Q5TAX3,Q12764, Q5TAX2, Q5TAX4, Q86XZ3, A2RRP0, B7Z8J5 D3DQ35,
UniProt Related Accession:	Q5TAX3
Molecular Weight:	1644
NCBI Full Name:	Terminal uridylyltransferase 4
NCBI Synonym Full Names:	zinc finger, CCHC domain containing 11
NCBI Official Symbol:	ZCCHC11
NCBI Official Synonym Symbols:	TUT4; PAPD3
NCBI Protein Information:	terminal uridylyltransferase 4; TUTase 4; PAP associated domain containing 3; zinc finger CCHC domain-containing protein 11
UniProt Protein Name:	Terminal uridylyltransferase 4
UniProt Synonym Protein Names:	Zinc finger CCHC domain-containing protein 11
Protein Family:	Terminal uridylyltransferase
UniProt Gene Name:	ZCCHC11
UniProt Entry Name:	TUT4_HUMAN

	Western blot analysis of extracts of 293T cells, using ZCCHC11 Antibody (CAB5972) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s.
	Immunofluorescence analysis of U2OS cells using ZCCHC11 Rabbit pAb (CAB5972) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using ZCCHC11 Rabbit pAb (CAB5972) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of H9C2 cells using ZCCHC11 Rabbit pAb (CAB5972) at dilution of 1:100. Blue: DAPI for nuclear staining.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)