The 5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit is specifically designed for the accurate and precise detection of 5-HIAA levels in various biological samples including urine, plasma, and cerebrospinal fluid. This kit offers high sensitivity and specificity, ensuring reliable and reproducible results for a wide range of research applications.5-HIAA is a metabolite of serotonin and is commonly used as a biomarker for various neurological and psychiatric disorders, including carcinoid tumors and serotonin syndrome. Monitoring 5-HIAA levels can provide valuable insights into the serotonin pathway and help in the diagnosis and treatment of these conditions.With its easy-to-use protocol and excellent performance characteristics, the 5-HIAA ELISA Kit is a valuable tool for researchers and healthcare professionals studying serotonin metabolism and related disorders.
Product Name:
5-HIAA (5-Hydroxyindoleacetic Acid) ELISA Kit
SKU:
UNES00048
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
