The 8-OHdG (8-hydroxydeoxyguanosine) ELISA Kit is specifically designed for the accurate quantification of 8-OHdG levels in biological samples such as urine, plasma, serum, and tissue homogenates. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.8-OHdG is a well-known marker of oxidative DNA damage and is commonly used in studying oxidative stress-related diseases such as cancer, cardiovascular diseases, and aging. By accurately measuring 8-OHdG levels, researchers can gain valuable insights into the pathogenesis of these diseases and potentially develop novel therapeutic strategies. Overall, the 8-OHdG ELISA Kit provides researchers with a powerful tool for investigating oxidative stress, biomarker discovery, and drug development in a reliable and efficient manner.
Product Name:
8-OHdG (8-Hydroxydeoxyguanosine) ELISA Kit
SKU:
UNES00018
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
