General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit
The General 8-hydroxydeoxyguanosine (8-OHdG) ELISA Kit is a reliable tool for the quantification of oxidative DNA damage in various biological samples. This kit offers high sensitivity and specificity, allowing for accurate and reproducible results in serum, plasma, and urine samples. 8-OHdG is a biomarker of oxidative stress and has been associated with various health conditions, including cancer, cardiovascular diseases, and aging.
By measuring 8-OHdG levels, researchers can gain insight into the extent of oxidative DNA damage within cells and tissues, providing valuable information for disease diagnosis and treatment monitoring. With its user-friendly protocol and robust performance, the General 8-OHdG ELISA Kit is an essential tool for researchers studying oxidative stress-related diseases and exploring potential therapeutic strategies.
Product Name:
General 8-Hydroxydeoxyguanosine (8-OHdG) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
84-99
91
EDTA plasma (n=5)
82-103
92
Heparin plasma (n=5)
84-102
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
