The AA (Arachidonic Acid) ELISA Kit from Assay Genie is a powerful tool for researchers looking to accurately quantify levels of arachidonic acid in biological samples. The kit is designed for use with human serum, plasma, and cell culture supernatants, offering high sensitivity and specificity for reliable and reproducible results. Arachidonic acid is a key component of cell membranes and serves as a precursor for the synthesis of important signaling molecules involved in inflammation and immune response.
Dysregulation of arachidonic acid metabolism has been linked to various diseases, including inflammation, cardiovascular disorders, and cancer. By utilizing the AA (Arachidonic Acid) ELISA Kit, researchers can gain valuable insights into the role of arachidonic acid in physiological and pathological processes, paving the way for the development of targeted therapeutic interventions. Trust Assay Genie for precise and accurate detection of arachidonic acid levels in your research studies.
Product Name:
AA/Arachidonic Acid ELISA Kit
SKU:
UNFI0042
Reactivity:
Universal
Assay Type:
Competitive ELISA, Coated with Antigen
Sensitivity:
0.938 ug/mL
Range:
1.563-100 ug/mL
Sample Type:
Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples
Storage:
2-8°C(Sealed), Don't cryopreserve.
Linearity:
Sample
1:2
1:4
1:8
Serum (n = 10)
85-99%
88-96%
88-102%
EDTA Plasma (n = 10)
86-99%
90-98%
83-99%
Heparin Plasma (n = 10)
86-99%
83-98%
87-99%
Recovery:
Sample
Recovery Range (%)
Average (%)
Serum (n = 10)
92-103
95
EDTA Plasma (n = 10)
85-100
94
Heparin Plasma (n = 10)
85-101
98
Note: The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
Step
Procedure
1
Reagent & Plate Preparation: Equilibrate TMB substrate for 30 minutes at room temperature. Prepare standards, samples (minimum 1:2 dilution), blanks, assign wells, and pre-wash the plate twice.
2
Sample & Biotin-Antibody Binding: Add 50 µL standard or sample followed by 50 µL biotin-labeled antibody to each well. Mix gently and incubate at 37°C for 45 minutes.
3
Washing: Wash the plate 5 times with wash buffer, allowing 1 minute soak time per wash.
4
Color Development: Add TMB substrate and incubate in the dark at 37°C for 10-20 minutes until color develops.
5
Stop Reaction: Add stop solution to terminate the reaction. The color changes from blue to yellow immediately.
6
Reading: Measure absorbance at 450 nm using a microplate reader.
Sample Type
Protocol
Serum
Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant and store appropriately.
Plasma
Collect using EDTA anticoagulant, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma.
Cell Culture Supernatant
Centrifuge at 1000 × g for 20 minutes at 4°C and collect clarified supernatant.
Cell Lysate
Lyse cells using recommended lysis buffer with protease inhibitors, centrifuge at 10,000 rpm for 10 minutes, and collect protein supernatant.
Tissue Homogenate
Homogenize tissue in PBS with protease inhibitors, centrifuge at 5000 × g for 5 minutes, and collect supernatant.
Other Sample Types
Centrifuge samples at 1000 × g for 15 minutes at 2–8°C and collect supernatant. For additional guidance, please contact techsupport@assaygenie.com.
Component
Quantity
Storage
48T
96T
ELISA Microplate (Dismountable)
8×6
8×12
Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Lyophilized Standard
1 vial
2 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
Biotin-labeled Antibody (Lyophilized)
1 vial
1 vial
Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C.
