The Arachidonic Acid ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of arachidonic acid levels in biological samples. Arachidonic acid is an important fatty acid involved in various physiological processes, including inflammation, blood clotting, and cell signaling.This kit provides researchers with a reliable and reproducible method for measuring arachidonic acid levels in serum, plasma, and cell culture supernatants. By accurately determining arachidonic acid levels, researchers can gain valuable insights into the role of this fatty acid in various diseases and biological processes. Whether studying inflammation, cardiovascular diseases, or neurological disorders, the Arachidonic Acid ELISA Kit is a valuable tool for researchers looking to further their understanding of arachidonic acid and its impact on human health. Get accurate and reliable results with this easy-to-use kit for a wide range of research applications.
Product Name:
AA (Arachidonic Acid) ELISA Kit
SKU:
UNES00032
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
0.94 ng/mL
Detection range:
1.56-100 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
