null

ABCC2 Rabbit Polyclonal Antibody (CAB8405)

SKU:
CAB8405
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:ABCC2 Rabbit Polyclonal Antibody
SKU:CAB8405
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-315 of human MRP2/ABCC2 (NP_000383.1).
Sequence:IFSA FSEN NESS NNPS SIAS FLSS ITYS WYDS IILK GYKR PLTL EDVW EVDE EMKT KTLV SKFE THMK RELQ KARR ALQR RQEK SSQQ NSGA RLPG LNKN QSQS QDAL VLED VEKK KKKS GTKK DVPK SWLM KALF
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:1000 IF/ICC,1:50 - 1:200
Synonyms:DJS; MRP2; cMRP; ABC30; CMOAT; MRP2/ABCC2
Positive Sample:HepG2,A549,Mouse lung,Rat lung,Rat kidney
Conjugate:Unconjugated
Cellular Localization:Apical cell membrane, Multi-pass membrane protein.
Calculated MW:174kDa
Observed MW:200kDa-270kDa

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein is expressed in the canalicular (apical) part of the hepatocyte and functions in biliary transport. Substrates include anticancer drugs such as vinblastine; therefore, this protein appears to contribute to drug resistance in mammalian cells. Several different mutations in this gene have been observed in patients with Dubin-Johnson syndrome (DJS), an autosomal recessive disorder characterized by conjugated hyperbilirubinemia.

Purification Method:Affinity purification
Gene ID:1244
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using MRP2/ABCC2 antibody (CAB8405) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

Related Products