Anti-ACE Antibody (CAB2805)

Antibody Name:	Anti-ACE Antibody
Antibody SKU:	CAB2805
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-270 of human ACE (NP_690043.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Human plasma, Mouse testis, Mouse kidney, Rat testis

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 30-270 of human ACE (NP_690043.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EASQ QVTV THGT SSQA TTSS QTTT HQAT AHQT SAQS PNLV TDEA EASK FVEE YDRT SQVV WNEY AEAN WNYN TNIT TETS KILL QKNM QIAN HTLK YGTQ ARKF DVNQ LQNT TIKR IIKK VQDL ERAA LPAQ ELEE YNKI LLDM ETTY SVAT VCHP NGSC LQLE PDLT NVMA TSRK YEDL LWAW EGWR DKAG RAIL QFYP KYVE LINQ AARL NGYV DAGD SWRS MYET PSLE QDLE RLFQ E
Gene ID:	1636
Uniprot:	P12821
Cellular Location:	Cell membrane, Cytoplasm, Secreted, Single-pass type I membrane protein
Calculated MW:	78kDa/83kDa/131kDa/149kDa
Observed MW:	110KDa/190KDa

Synonyms:

ACE, ACE1, CD143, DCP, DCP1, angiotensin-converting enzyme, ICH, MVCD3

Background:

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active.

UniProt Protein Function:	ACE: Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Genetic variations in ACE may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Defects in ACE are a cause of renal tubular dysgenesis (RTD). RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Genetic variations in ACE are associated with susceptibility to microvascular complications of diabetes type 3 (MVCD3). These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new- onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Defects in ACE are a cause of susceptibility to intracerebral hemorrhage (ICH). A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Belongs to the peptidase M2 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:EC 3.4.15.1; Protease; Membrane protein, integral Chromosomal Location of Human Ortholog: 17q23.3 Cellular Component: extracellular space; lysosome; extracellular region; integral to membrane; plasma membrane; endosome; external side of plasma membrane Molecular Function:tripeptidyl-peptidase activity; peptidyl-dipeptidase activity; protein binding; metallopeptidase activity; carboxypeptidase activity; zinc ion binding; mitogen-activated protein kinase kinase binding; endopeptidase activity; bradykinin receptor binding; exopeptidase activity; mitogen-activated protein kinase binding; drug binding; actin binding; chloride ion binding Biological Process: mononuclear cell proliferation; regulation of vasodilation; angiotensin mediated regulation of renal output; neutrophil mediated immunity; regulation of angiotensin metabolic process; angiotensin maturation; arachidonic acid secretion; proteolysis; regulation of systemic arterial blood pressure by renin-angiotensin; antigen processing and presentation of peptide antigen via MHC class I; regulation of smooth muscle cell migration; cellular protein metabolic process; heart contraction; regulation of blood pressure; peptide catabolic process; beta-amyloid metabolic process; regulation of vasoconstriction; angiotensin catabolic process in blood; spermatogenesis; blood vessel remodeling; hormone catabolic process; kidney development Disease: Microvascular Complications Of Diabetes, Susceptibility To, 3; Renal Tubular Dysgenesis; Alzheimer Disease; Hemorrhage, Intracerebral, Susceptibility To
NCBI Summary:	This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]
UniProt Code:	P12821
NCBI GenInfo Identifier:	113045
NCBI Gene ID:	1636
NCBI Accession:	P12821.1
UniProt Secondary Accession:	P12821,P22966, Q53YX9, Q59GY8, Q7M4L4, B0LPF0, B4DXI3 E7EU16,
UniProt Related Accession:	P12821
Molecular Weight:	1306
NCBI Full Name:	Angiotensin-converting enzyme
NCBI Synonym Full Names:	angiotensin I converting enzyme
NCBI Official Symbol:	ACE
NCBI Official Synonym Symbols:	DCP; ICH; ACE1; DCP1; CD143; MVCD3
NCBI Protein Information:	angiotensin-converting enzyme; kininase II; peptidase P; CD143 antigen; testicular ECA; carboxycathepsin; dipeptidyl carboxypeptidase 1; dipeptidyl carboxypeptidase I; angiotensin converting enzyme, somatic isoform; angiotensin I converting enzyme (peptidyl-dipeptidase A) 1; angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript
UniProt Protein Name:	Angiotensin-converting enzyme
UniProt Synonym Protein Names:	Dipeptidyl carboxypeptidase I; Kininase II; CD_antigen: CD143Angiotensin-converting enzyme, soluble form
Protein Family:	Acetylcholinesterase
UniProt Gene Name:	ACE
UniProt Entry Name:	ACE_HUMAN

	Western blot analysis of extracts of Human plasma, using ACE antibody (CAB2805) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts of various cell lines, using ACE antibody (CAB2805) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Immunohistochemistry of paraffin-embedded rat lung using ACE Rabbit pAb (CAB2805) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using ACE Rabbit pAb (CAB2805) at dilution of 1:100 (40x lens).