Acetyl-Histone H3-K4 Rabbit Polyclonal Antibody (CAB17019)

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SKU:
CAB17019
€99 - €249

Description

Technical Manual

Acetyl-Histone H3-K4 Rabbit Polyclonal Antibody

Product Name:Acetyl-Histone H3-K4 Rabbit Polyclonal Antibody
Product SKU:CAB17019
Size:20uL, 50 uL
Host Species:Rabbit
Purification Method:Affinity purification
Isotype:IgG
Background:

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen:A synthetic acetylated peptide around K4 of human Histone H3 (NP_003484.1).
Sequence:

ARTKQ

Gene ID:8290
Swiss Prot:Q16695
Synonyms:H3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW:15kDa
Observed MW:17kDa
Reactivity:Human
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting 
Positive Samples:HeLa
Cellular Location:Chromosome, Nucleus

Product Images

Acetyl-Histone H3-K4 Rabbit Polyclonal Antibody (CAB17019)

Western blot - Acetyl-Histone H3-K4 Rabbit pAb (CAB17019)

Uniprot Information

UniProt Protein Function:HIST3H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Expressed in testicular cells. Belongs to the histone H3 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 1q42

Cellular Component: nucleoplasm; nucleus

Molecular Function:protein binding; DNA binding; histone binding; protein heterodimerization activity

Biological Process: nucleosome assembly; negative regulation of protein oligomerization; protein heterotetramerization; telomere maintenance

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008]
UniProt Code:Q16695
NCBI GenInfo Identifier:18202512
NCBI Gene ID:8290
NCBI Accession:Q16695.3
UniProt Secondary Accession:Q16695,Q6FGU4, B2R5K3,
UniProt Related Accession:Q16695
Molecular Weight:
NCBI Full Name:Histone H3.1t
NCBI Synonym Full Names:histone cluster 3, H3
NCBI Official Symbol:HIST3H3 
NCBI Official Synonym Symbols:H3t; H3.4; H3/g; H3FT 
NCBI Protein Information:histone H3.1t; H3/t; histone 3, H3; H3 histone family, member T
UniProt Protein Name:Histone H3.1t
UniProt Synonym Protein Names:H3/g
UniProt Gene Name:HIST3H3 
UniProt Entry Name:H31T_HUMAN
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Additional Information

Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
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