The Acetyl-Histone H4-K91 Antibody (CAB7409) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, raised in rabbits, specifically targets the acetylation of histone H4 at lysine 91, a critical post-translational modification involved in gene regulation and chromatin remodeling.Histone acetylation at lysine 91 of histone H4 has been linked to various biological processes, including DNA repair, transcriptional activation, and cell cycle regulation. The CAB7409 antibody has been validated for use in Western blot applications, enabling detection and analysis of acetylated histone H4 (K91) in various cell types and tissue samples.
This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat, Other (Wide Range Predicted) samples.
Product Name:
Acetyl-Histone H4-K91 Antibody
SKU:
CAB7409
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat, Other (Wide Range Predicted)
Conjugate:
Unconjugated
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Sequence:
ALKR Q
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB
1:500 - 1:1000
IF/ICC
1:50 - 1:200
ELISA
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Purification Method
Affinity purification
Gene ID
8359
RRID
AB_2767940
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H4-K91 Rabbit pAb (CAB7409) at 1:1000 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 300s.
Immunofluorescence analysis of C6 cells using Acetyl-Histone H4-K91 Rabbit pAb (CAB7409) at dilution of 1:100.C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H4-K91 Rabbit pAb (CAB7409) at dilution of 1:100.NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
