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Anti-ACTA2/ACTG2 Antibody (MACO0472)

SKU:
MACO0472
Product Type:
Antibody
Reactivity:
Human
Host Species:
Mouse
Isotype:
IgG2a, Kappa
Applications:
ELISA
IHC
Antibody Type:
Monoclonal Antibody
Conjugation:
Unconjugated
€339
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Description

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Product Name:Anti-ACTA2/ACTG2 Antibody
Product Sku:MACO0472
Size:50ul
Host Species:Mouse
Tested Applications:ELISA, IHC
Recommended Dilutions:IHC:1:20-1:200
Species Reactivity:Human
Immunogen:Synthesized peptide derived from human Actin, smooth muscle (SMA)
Form:Liquid
Storage Buffer:Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Clonality:Monoclonal
Isotype:IgG2a, Kappa
Conjugate:Non-conjugated
Background:Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Synonyms:Actin, aortic smooth muscle (Alpha-actin-2) (Cell growth-inhibiting gene 46 protein) [Cleaved into: Actin, aortic smooth muscle, intermediate form], ACTA2, ACTSA ACTVS
Product ImageIHC image of MACO0472 diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-mouse IgG polymer labeled by HRP and visualized using 0.05% DAB.
UniProt Protein Function:ACTA2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Up-regulated in response to enterovirus 71 (EV71) infection. Belongs to the actin family.
UniProt Protein Details:

Protein type:Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 10q23.3

Cellular Component: extracellular space; smooth muscle contractile fiber; protein complex; cytoplasm; cytosol; actin cytoskeleton

Molecular Function:protein kinase binding; ATP binding

Biological Process: muscle contraction; response to virus; regulation of blood pressure; vascular smooth muscle contraction

Disease: Moyamoya Disease 5; Multisystemic Smooth Muscle Dysfunction Syndrome; Aortic Aneurysm, Familial Thoracic 6

NCBI Summary:The protein encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Defects in this gene cause aortic aneurysm familial thoracic type 6. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2008]
UniProt Code:P62736
NCBI GenInfo Identifier:51316972
NCBI Gene ID:59
NCBI Accession:P62736.1
UniProt Secondary Accession:P62736,P03996, P04108, Q6FI19, B2R8A4,
UniProt Related Accession:P62736
Molecular Weight:42,009 Da
NCBI Full Name:Actin, aortic smooth muscle
NCBI Synonym Full Names:actin, alpha 2, smooth muscle, aorta
NCBI Official Symbol:ACTA2  
NCBI Official Synonym Symbols:AAT6; ACTSA; MYMY5  
NCBI Protein Information:actin, aortic smooth muscle; alpha-cardiac actin; cell growth-inhibiting gene 46 protein
UniProt Protein Name:Actin, aortic smooth muscle
UniProt Synonym Protein Names:Alpha-actin-2; Cell growth-inhibiting gene 46 protein
Protein Family:Actin
UniProt Gene Name:ACTA2  
UniProt Entry Name:ACTA_HUMAN

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