The ADMA (Asymmetric Dimethylarginine) ELISA Kit is specially designed for the precise measurement of ADMA levels in biological samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research applications.ADMA is an important metabolite that inhibits nitric oxide production and plays a role in cardiovascular diseases, kidney dysfunction, and other health conditions. By detecting ADMA levels, researchers can better understand the mechanisms underlying these diseases and potentially identify new therapeutic targets.Overall, the ADMA ELISA Kit from Assay Genie provides researchers with a valuable tool for studying the role of ADMA in various pathological conditions and developing strategies for disease diagnosis and treatment.
Product Name:
ADMA (Asymmetrical Dimethylarginine) ELISA Kit
SKU:
UNES00027
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Competitive
Assay type:
Competitive-ELISA
Assay time:
2 h 30 min
Sensitivity:
9.38 ng/mL
Detection range:
15.63-1000 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with the target antigen. Standards or samples are added along with a biotinylated detection antibody. The target antigen present in the sample competes with the immobilized antigen for binding to the detection antibody. After incubation, Avidin-Horseradish Peroxidase (HRP) conjugate is added. Free components are washed away. The substrate solution is then added, resulting in a color change. The intensity of the color is inversely proportional to the concentration of the target antigen in the sample. The reaction is stopped by the addition of stop solution, and the color changes from blue to yellow. The optical density (OD) is measured at 450 nm ± 2 nm. The concentration of the target protein is calculated by comparing the OD values of the samples to the standard curve.
