Anti-AKR1C2 Antibody (CAB1048)

Antibody Name:	Anti-AKR1C2 Antibody
Antibody SKU:	CAB1048
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C2 (NP_995317.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, A549, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human AKR1C2 (NP_995317.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDSK YQCV KLND GHFM PVLG FGTY APAE VPKS KALE AVKL AIEA GFHH IDSA HVYN NEEQ VGLA IRSK IADG SVKR EDIF YTSK LWSN SHRP ELVR PALE RSLK NLQL DYVD LYLI HFPV SVKP GEEV IPKD ENGK ILFD TVDL CATW EAME KCKD AGLA KSIG VSNF NHRL LEMI LNKP GLKY KPVC NQVE CHPY FNQR KLLD FCKS KDIV LVAY SALG SHRE EPWV DPNS PVLL EDPV LCAL AKKH KRTP ALIA LRYQ LQRG VVVL AKSY NEQR IRQN VQVF EFQL TSEE MKAI DGLN RNVR YLTL DIFA GPPN YPFS DEY
Gene ID:	1646
Uniprot:	P52895
Cellular Location:	Cytoplasm
Calculated MW:	15kDa/36kDa
Observed MW:	37kDa

Synonyms:

AKR1C2, AKR1C-pseudo, BABP, DD, DD-2, DD/BABP, DD2, DDH2, HAKRD, HBAB, MCDR2, SRXY8, TDD

Background:

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene.

UniProt Protein Function:	AKR1C2: Works in concert with the 5-alpha/5-beta-steroid reductases to convert steroid hormones into the 3-alpha/5-alpha and 3-alpha/5-beta-tetrahydrosteroids. Catalyzes the inactivation of the most potent androgen 5-alpha-dihydrotestosterone (5-alpha- DHT) to 5-alpha-androstane-3-alpha,17-beta-diol (3-alpha-diol). Has a high bile-binding ability. Defects in AKR1C2 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. Belongs to the aldo/keto reductase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Xenobiotic Metabolism - metabolism by cytochrome P450; EC 1.3.1.20; EC 1.1.1.357; Oxidoreductase Chromosomal Location of Human Ortholog: 10p15-p14 Cellular Component: cytoplasm Molecular Function:oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; ketosteroid monooxygenase activity; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase activity; carboxylic acid binding; bile acid binding; aldehyde reductase activity; phenanthrene 9,10-monooxygenase activity Biological Process: steroid metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of protein kinase B signaling cascade; epithelial cell differentiation; digestion; positive regulation of cell proliferation; progesterone metabolic process; prostaglandin metabolic process Disease: 46,xy Sex Reversal 8
NCBI Summary:	This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols using NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme binds bile acid with high affinity, and shows minimal 3-alpha-hydroxysteroid dehydrogenase activity. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
UniProt Code:	P52895
NCBI GenInfo Identifier:	20532374
NCBI Gene ID:	1646
NCBI Accession:	P52895.3
UniProt Secondary Accession:	P52895,Q14133, Q5SR16, Q7M4N1, Q96A71, A8K2N9, B4DKR9
UniProt Related Accession:	P52895
Molecular Weight:	15,748 Da
NCBI Full Name:	Aldo-keto reductase family 1 member C2
NCBI Synonym Full Names:	aldo-keto reductase family 1, member C2
NCBI Official Symbol:	AKR1C2
NCBI Official Synonym Symbols:	DD; DD2; TDD; BABP; DDH2; HBAB; HAKRD; MCDR2; SRXY8; AKR1C-pseudo
NCBI Protein Information:	aldo-keto reductase family 1 member C2; DD-2; DD/BABP; 3-alpha-HSD3; pseudo-chlordecone reductase; type II dihydrodiol dehydrogenase; chlordecone reductase homolog HAKRD; testicular 17,20-desmolase deficiency; trans-1,2-dihydrobenzene-1,2-diol dehydrogenase; dihydrodiol dehydrogenase 2; bile acid binding protein; 3-alpha hydroxysteroid dehydrogenase, type III
UniProt Protein Name:	Aldo-keto reductase family 1 member C2
UniProt Synonym Protein Names:	3-alpha-HSD3; Chlordecone reductase homolog HAKRD; Dihydrodiol dehydrogenase 2; DD-2; DD2; Dihydrodiol dehydrogenase/bile acid-binding protein; DD/BABP; Trans-1,2-dihydrobenzene-1,2-diol dehydrogenase (EC:1.3.1.20); Type III 3-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.357)
Protein Family:	Aspartate-semialdehyde dehydrogenase
UniProt Gene Name:	AKR1C2
UniProt Entry Name:	AK1C2_HUMAN

	Western blot analysis of extracts of various cell lines, using AKR1C2 antibody (CAB1048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of L929 cells using AKR1C2 Polyclonal Antibody (CAB1048) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U-2 OS cells using AKR1C2 Polyclonal Antibody (CAB1048) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
AKR1C2 Antibody (PACO02570)	Human Aldo-keto reductase family 1 member C2 (AKR1C2) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)