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ALDH1A1 Rabbit Monoclonal Antibody (CAB0157)

SKU:
CAB0157
Product Type:
Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:ALDH1A1 Rabbit Monoclonal Antibody
SKU:CAB0157
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 250-350 of human ALDH1A1 (NP_000680.2).
Sequence:VGKL IKEA AGKS NLKR VTLE LGGK SPCI VLAD ADLD NAVE FAHH GVFY HQGQ CCIA ASRI FVEE SIYD EFVR RSVE RAKK YILG NPLT PGVT QGPQ IDKE Q
Tested Applications:WB IF/ICC IP ELISA
Recommended Dilution:WB,1:2000 - 1:20000 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:ALDC; ALDH1; HEL-9; HEL12; PUMB1; ALDH11; RALDH1; ALDH-E1; HEL-S-53e; ALDH1A1
Positive Sample:A549,Hep G2,Mouse lung,Mouse liver,Mouse testis,Rat lung,Rat kidney,Rat testis
Conjugate:Unconjugated
Cellular Localization:Cytoplasm.
Calculated MW:55kDa
Observed MW:55kDa

The protein encoded by this gene belongs to the aldehyde dehydrogenase family. Aldehyde dehydrogenase is the next enzyme after alcohol dehydrogenase in the major pathway of alcohol metabolism. There are two major aldehyde dehydrogenase isozymes in the liver, cytosolic and mitochondrial, which are encoded by distinct genes, and can be distinguished by their electrophoretic mobility, kinetic properties, and subcellular localization. This gene encodes the cytosolic isozyme. Studies in mice show that through its role in retinol metabolism, this gene may also be involved in the regulation of the metabolic responses to high-fat diet.

Purification Method:Affinity purification
Gene ID:216
Clone Number:ARC52440
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates, using ALDH1A1 Rabbit mAb (CAB0157) at 1:20000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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